CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody-Antigen Interface.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2017.05.011
Abstract: Structure-based vaccine design depends on extensive structural analyses of antigen-antibody complexes.Single-particle electron cryomicroscopy (cryoEM) can circumvent some of the problems of x-ray crystallography as a pipeline for obtaining the required structures. We have examined the… read more here.
Keywords: antibody; influenza virus; cryoem; structure ... See more keywords
Microscopic charge fluctuations cause minimal contrast loss in cryoEM
Sign Up to like & getrecommendations! Published in 2018 at "Ultramicroscopy"
DOI: 10.1016/j.ultramic.2018.01.011
Abstract: Highlights • A physical account of charge fluctuations in low-dose cryoEM is presented.• We quantify the bee swarm charging phenomenon on cryoEM specimen.• We measure the envelope function caused by charge fluctuations.• The effects of… read more here.
Keywords: cryoem; cause minimal; fluctuations cause; charge fluctuations ... See more keywords
Enabling a Paradigm Shift in CryoEM Sample Preparation with chameleon
Sign Up to like & getrecommendations! Published in 2021 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927621002336
Abstract: The period responsible for the current generation of cryogenic electron microscopy (cryo-EM) instrumentation and image processing software is often referred to as the “resolution revolution.” The results of these technological advancements have been achievements in… read more here.
Keywords: paradigm shift; microscopy; cryoem; enabling paradigm ... See more keywords
Deep learning based mixed-dimensional GMM for characterizing variability in CryoEM
Sign Up to like & getrecommendations! Published in 2021 at "Nature methods"
DOI: 10.1038/s41592-021-01220-5
Abstract: Structural flexibility and/or dynamic interactions with other molecules is a critical aspect of protein function. CryoEM provides direct visualization of individual macromolecules sampling different conformational and compositional states. While numerous methods are available for computational… read more here.
Keywords: cryoem; deep learning; mixed dimensional; based mixed ... See more keywords
Maintaining the momentum in cryoEM for biological discovery.
Sign Up to like & getrecommendations! Published in 2022 at "Faraday discussions"
DOI: 10.1039/d2fd00129b
Abstract: Cryo-electron microscopy (cryoEM) has been transformed over the last decade, with continual new hardware and software tools coming online, pushing the boundaries of what is possible and the nature and complexity of projects that can… read more here.
Keywords: maintaining momentum; biological discovery; microscopy; cryoem biological ... See more keywords
Fully automated multi-grid cryoEM screening using Smart Leginon
Sign Up to like & getrecommendations! Published in 2022 at "IUCrJ"
DOI: 10.1101/2022.07.23.501225
Abstract: Single particle cryo-electron microscopy (cryoEM) is a swiftly growing method for understanding protein structure. With increasing demand for high-throughput, high-resolution cryoEM services comes greater demand for rapid and automated cryoEM grid and sample screening. During… read more here.
Keywords: fully automated; leginon; collection; multi grid ... See more keywords
Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme
Sign Up to like & getrecommendations! Published in 2018 at "eLife"
DOI: 10.7554/elife.33572
Abstract: Insulin degrading enzyme (IDE) plays key roles in degrading peptides vital in type two diabetes, Alzheimer's, inflammation, and other human diseases. However, the process through which IDE recognizes peptides that tend to form amyloid fibrils… read more here.
Keywords: insulin; cryoem; degrading enzyme; insulin degrading ... See more keywords
Routine single particle CryoEM sample and grid characterization by tomography
Sign Up to like & getrecommendations! Published in 2018 at "eLife"
DOI: 10.7554/elife.34257
Abstract: Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50… read more here.
Keywords: sample; cryoem; particle; single particle ... See more keywords