CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody-Antigen Interface.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2017.05.011
Abstract: Structure-based vaccine design depends on extensive structural analyses of antigen-antibody complexes.Single-particle electron cryomicroscopy (cryoEM) can circumvent some of the problems of x-ray crystallography as a pipeline for obtaining the required structures. We have examined the… read more here.
Keywords: antibody; influenza virus; cryoem; structure ... See more keywords
Microscopic charge fluctuations cause minimal contrast loss in cryoEM
Sign Up to like & getrecommendations! Published in 2018 at "Ultramicroscopy"
DOI: 10.1016/j.ultramic.2018.01.011
Abstract: Highlights • A physical account of charge fluctuations in low-dose cryoEM is presented.• We quantify the bee swarm charging phenomenon on cryoEM specimen.• We measure the envelope function caused by charge fluctuations.• The effects of… read more here.
Keywords: cryoem; cause minimal; fluctuations cause; charge fluctuations ... See more keywords
Enabling a Paradigm Shift in CryoEM Sample Preparation with chameleon
Sign Up to like & getrecommendations! Published in 2021 at "Microscopy and Microanalysis"
DOI: 10.1017/s1431927621002336
Abstract: The period responsible for the current generation of cryogenic electron microscopy (cryo-EM) instrumentation and image processing software is often referred to as the “resolution revolution.” The results of these technological advancements have been achievements in… read more here.
Keywords: paradigm shift; microscopy; cryoem; enabling paradigm ... See more keywords
CryoEM structures reveal how the bacterial flagellum rotates and switches direction
Sign Up to like & getrecommendations! Published in 2024 at "Nature Microbiology"
DOI: 10.1038/s41564-024-01674-1
Abstract: Bacterial chemotaxis requires bidirectional flagellar rotation at different rates. Rotation is driven by a flagellar motor, which is a supercomplex containing multiple rings. Architectural uncertainty regarding the cytoplasmic C-ring, or ‘switch’, limits our understanding of… read more here.
Keywords: direction; reveal bacterial; cryoem; structures reveal ... See more keywords
Deep learning based mixed-dimensional GMM for characterizing variability in CryoEM
Sign Up to like & getrecommendations! Published in 2021 at "Nature methods"
DOI: 10.1038/s41592-021-01220-5
Abstract: Structural flexibility and/or dynamic interactions with other molecules is a critical aspect of protein function. CryoEM provides direct visualization of individual macromolecules sampling different conformational and compositional states. While numerous methods are available for computational… read more here.
Keywords: cryoem; deep learning; mixed dimensional; based mixed ... See more keywords
Maintaining the momentum in cryoEM for biological discovery.
Sign Up to like & getrecommendations! Published in 2022 at "Faraday discussions"
DOI: 10.1039/d2fd00129b
Abstract: Cryo-electron microscopy (cryoEM) has been transformed over the last decade, with continual new hardware and software tools coming online, pushing the boundaries of what is possible and the nature and complexity of projects that can… read more here.
Keywords: maintaining momentum; biological discovery; microscopy; cryoem biological ... See more keywords
Outrunning protein diffusion to the air–water interface in cryoEM
Sign Up to like & getrecommendations! Published in 2025 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2516900122
Abstract: Here, we report a series of measurements indicating that it is physically possible to thin and vitrify a specimen for electron cryomicroscopy (cryoEM) faster than proteins diffuse to the air–water interface. We achieved this by… read more here.
Keywords: air water; cryoem; water interface;
Fully automated multi-grid cryoEM screening using Smart Leginon
Sign Up to like & getrecommendations! Published in 2022 at "IUCrJ"
DOI: 10.1101/2022.07.23.501225
Abstract: Single particle cryo-electron microscopy (cryoEM) is a swiftly growing method for understanding protein structure. With increasing demand for high-throughput, high-resolution cryoEM services comes greater demand for rapid and automated cryoEM grid and sample screening. During… read more here.
Keywords: fully automated; leginon; collection; multi grid ... See more keywords
Crystallographic and cryoEM analyses reveal SARS-CoV-2 SL5 is a mobile T-shaped four-way junction with deep pockets.
Sign Up to like & getrecommendations! Published in 2025 at "RNA"
DOI: 10.1261/rna.080413.125
Abstract: Stem-loop 5 (SL5) is a structural element that is conserved across coronavirus genomic RNAs. It spans the start codon from which the long ORF1 is translated in full-length viral RNA. Phylogenetic conservation indicates that it… read more here.
Keywords: four way; junction; way junction; cryoem ... See more keywords
Mitigating the Blurring Effect of CryoEM Averaging on a Flexible and Highly Symmetric Protein Complex through Sub-Particle Reconstruction
Sign Up to like & getrecommendations! Published in 2024 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms25115665
Abstract: Many macromolecules are inherently flexible as a feature of their structure and function. During single-particle CryoEM processing, flexible protein regions can be detrimental to high-resolution reconstruction as signals from thousands of particles are averaged together.… read more here.
Keywords: blurring effect; protein; symmetric protein; particle ... See more keywords
Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme
Sign Up to like & getrecommendations! Published in 2018 at "eLife"
DOI: 10.7554/elife.33572
Abstract: Insulin degrading enzyme (IDE) plays key roles in degrading peptides vital in type two diabetes, Alzheimer's, inflammation, and other human diseases. However, the process through which IDE recognizes peptides that tend to form amyloid fibrils… read more here.
Keywords: insulin; cryoem; degrading enzyme; insulin degrading ... See more keywords