Minimizing the deleterious effects of endophytes in plant shoot tip cryopreservation
Sign Up to like & getrecommendations! Published in 2022 at "Applications in Plant Sciences"
DOI: 10.1002/aps3.11489
Abstract: Abstract Plant cryopreservation technologies are used within gene banks for the long‐term preservation of vegetatively propagated collections. Surface‐sterilized plant tissues grown in the field, greenhouse/screenhouse, growth chamber, or in vitro are the source of shoot… read more here.
Keywords: deleterious effects; shoot; plant; minimizing deleterious ... See more keywords
hMSCs in contact with DMSO for cryopreservation: Experiments and modeling of osmotic injury and cytotoxic effect
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28174
Abstract: In this study a combined analysis of osmotic injury and cytotoxic effect useful for the optimization of the cryopreservation process of a cell suspension is carried out. The case of human Mesenchymal Stem Cells (hMSCs)… read more here.
Keywords: cell; contact; injury cytotoxic; osmotic injury ... See more keywords
Self‐Pressurized Rapid Freezing as Cryo‐Fixation Method for Electron Microscopy and Cryopreservation of Living Cells
Sign Up to like & getrecommendations! Published in 2018 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.47
Abstract: Reduction or complete prevention of ice crystal formation during freezing of biological specimens is mandatory for two important biological applications: (1) cryopreservation of living cells or tissues for long‐term storage, and (2) cryo‐fixation for ultrastructural… read more here.
Keywords: cryopreservation living; cryo fixation; microscopy; cryopreservation ... See more keywords
Primary colonospheres maintain stem cell‐like key features after cryopreservation
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.29150
Abstract: The development of efficient and repeatable protocols for biobanking and prolonged storage of cancer stem cells (CSCs), with minimum alterations in biological function, is valuable and desired, particularly for retrospective analysis and clinical applications. In… read more here.
Keywords: cell like; primary colonospheres; stem cell; cryopreservation ... See more keywords
Low‐density lipoproteins protect sperm during cryopreservation in buffalo: Unraveling mechanism of action
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23434
Abstract: This study was carried out to reveal factors and the mechanism of action by which low‐density lipoproteins (LDLs) protect sperm better than egg yolk (EY) during cryopreservation. We extracted LDL from EY and compared the… read more here.
Keywords: extender; low density; cryopreservation; calcium ... See more keywords
Factors Affecting Human Umbilical Cord Blood Quality Prior to Cryopreservation: The Importance of Birth Weight and Gestational Age
Sign Up to like & getrecommendations! Published in 2019 at "STEM CELLS Translational Medicine"
DOI: 10.1002/sctm.12571
Abstract: 22 Introduction Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells and is useful for the treatment of blood diseases. The cost of UCB storage is high; thus, it is necessary to… read more here.
Keywords: quality; cord blood; blood; cryopreservation ... See more keywords
Cryopreservation of Algae.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0783-1_32
Abstract: Cryopreservation has been successfully used in the banking and maintenance of cultures of microorganisms, from bacteria to yeasts, since the onset of cryobiology. Biobanking of marine biological resources is crucial for development of scientific knowledge… read more here.
Keywords: biological resources; cryopreservation; biology; cryobiology ... See more keywords
Cryopreservation of Fern Spores and Pollen.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0783-1_33
Abstract: Fern spores and pollen are haploid plant germplasm of microscopic nature that can be used to regenerate full plants through germination (fern spores) or to fertilize seed-bearing plants through breeding programs (pollen). Due to their… read more here.
Keywords: desiccation; seed; spores pollen; fern spores ... See more keywords
Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0783-1_35
Abstract: Cryopreservation of shoot tips facilitates long-term storage of plant genetic resources which can otherwise only be propagated vegetatively. The vitrification approach using the cryoprotectant plant vitrification solution 3 (PVS3, 50% sucrose and 50% glycerol) is… read more here.
Keywords: plant; potato mint; cryopreservation; mint garlic ... See more keywords
Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0783-1_6
Abstract: From early dry-ice-based freezers and passive coolers, cryopreservation devices have come a long way. With increasing interest in the field of cryobiology from new scientific applications, the importance of reliable, traceable, and reproducible cold chain… read more here.
Keywords: control freezing; freezing technology; technology control; cryopreservation ... See more keywords
Appendix A: Cryotech® Vitrification Thawing.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6828-2_21
Abstract: In the last 15-20 years, many centers are using vitrification as a method of choice for cryopreservation of human oocytes and embryos. As vitrification technologies have improved their success profiles, new applications seem to have emerged,… read more here.
Keywords: vitrification thawing; appendix cryotech; method; cryotech vitrification ... See more keywords