Efficiency of mannitol-supplemented medium during adding/removing ovarian tissue with penetrating cryoprotective agents
Sign Up to like & getrecommendations! Published in 2017 at "Cell and Tissue Banking"
DOI: 10.1007/s10561-017-9623-8
Abstract: Most protocols to cryopreserve ovarian tissue utilize the permeable cryoprotective agents (CPAs) in 1.5 M concentration. However the issues related to the ability to use higher concentrations of CPAs have remained open. The research aim was… read more here.
Keywords: removing ovarian; ovarian tissue; adding removing; tissue ... See more keywords
Natural deep eutectic systems as alternative nontoxic cryoprotective agents.
Sign Up to like & getrecommendations! Published in 2018 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2018.06.010
Abstract: Natural deep eutectic systems (NADES) are mostly composed of natural primary metabolites such as sugars, sugar alcohols, organic acids, amino acids and amines. These simple molecules have been identified in animals living in environments with… read more here.
Keywords: systems alternative; natural deep; deep eutectic; eutectic systems ... See more keywords
Optimization of protocols for microinjection-based delivery of cryoprotective agents into Japanese whiting Sillago japonica embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2018.10.007
Abstract: Microinjection has proven useful for introduction of low-permeability cryoprotective agents (CPAs) into fish eggs or embryos for cryopreservation. In this work, we examined the suitable conditions for single or combined microinjection into the perivitelline space… read more here.
Keywords: embryos; japanese whiting; protocols microinjection; microinjection ... See more keywords
Polyampholytes as Emerging Macromolecular Cryoprotectants
Sign Up to like & getrecommendations! Published in 2019 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.9b01053
Abstract: Cellular cryopreservation is a platform technology which underpins cell biology, biochemistry, biomaterials, diagnostics, and the cold chain for emerging cell-based therapies. This technique relies on effective methods for banking and shipping to avoid the need… read more here.
Keywords: macromolecular cryoprotectants; polyampholytes emerging; biology; emerging macromolecular ... See more keywords
Loading equine oocytes with cryoprotective agents captured with a finite element method model
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-99287-9
Abstract: Cryopreservation can be used to store equine oocytes for extended periods so that they can be used in artificial reproduction technologies at a desired time point. It requires use of cryoprotective agents (CPAs) to protect… read more here.
Keywords: finite element; element method; model; equine oocytes ... See more keywords
Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents
Sign Up to like & getrecommendations! Published in 2020 at "BMC Biotechnology"
DOI: 10.1186/s12896-020-00636-9
Abstract: Background Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6–8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and metabolic… read more here.
Keywords: cell; cryopreservation; vitrification; stem cells ... See more keywords
Modification of cellular membranes conveys cryoprotection to cells during rapid, non-equilibrium cryopreservation
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0205520
Abstract: Rapid cooling and re-warming has been shown promising to cryopreserve living cells, which cannot be preserved by conventional slow freezing methods. However, success is limited by the cytotoxicity of highly concentrated cryoprotective agents. Recent results… read more here.
Keywords: cellular membranes; rapid non; non equilibrium; cryopreservation ... See more keywords
The Role of Cryoprotective Agents in Liposome Stabilization and Preservation
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232012487
Abstract: To improve liposomes’ usage as drug delivery vehicles, cryoprotectants can be utilized to prevent constituent leakage and liposome instability. Cryoprotective agents (CPAs) or cryoprotectants can protect liposomes from the mechanical stress of ice by vitrifying… read more here.
Keywords: stabilization preservation; cryoprotective agents; liposome stabilization; agents liposome ... See more keywords