Characterization and engineering of two new GH9 and GH48 cellulases from a Bacillus pumilus isolated from Lake Bogoria
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology Letters"
DOI: 10.1007/s10529-020-03056-z
Abstract: Objectives To search for new alkaliphilic cellulases and to improve their efficiency on crystalline cellulose through molecular engineering Results Two novel cellulases, Bp GH9 and Bp GH48, from a Bacillus pumilus strain were identified, cloned… read more here.
Keywords: bacillus pumilus; engineering; gh9 gh48; module ... See more keywords
Molecular insights on the crystalline cellulose-water interfaces via three-dimensional atomic force microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abq0160
Abstract: Cellulose, a renewable structural biopolymer, is ubiquitous in nature and is the basic reinforcement component of the natural hierarchical structures of living plants, bacteria, and tunicates. However, a detailed picture of the crystalline cellulose surface… read more here.
Keywords: crystalline cellulose; water; force microscopy; microscopy ... See more keywords
Bacterial crystalline cellulose secretion via a supramolecular BcsHD scaffold
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.add1170
Abstract: Cellulose, the most abundant biopolymer on Earth, is not only the predominant constituent of plants but also a key extracellular polysaccharide in the biofilms of many bacterial species. Depending on the producers, chemical modifications, and… read more here.
Keywords: bacterial crystalline; crystalline cellulose; crystalline; cellulose secretion ... See more keywords
X-Ray Scattering Reveals Two Mechanisms of Cellulose Microfibril Degradation by Filamentous Fungi
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DOI: 10.1128/aem.00995-22
Abstract: Cellulose degradation by fungi plays a fundamental role in terrestrial carbon cycling, but the mechanisms by which fungi cope with the crystallinity of cellulose are not fully understood. We used X-ray scattering to analyze how… read more here.
Keywords: crystalline cellulose; ray scattering; two mechanisms; degradation ... See more keywords
A Type IX Secretion System Substrate Involved in Crystalline Cellulose Degradation by Affecting Crucial Cellulose Binding Proteins in Cytophaga hutchinsonii
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DOI: 10.1128/aem.01837-21
Abstract: The widespread aerobic cellulolytic bacterium Cytophaga hutchinsonii, belonging to the phylum Bacteroidetes, utilizes a novel mechanism to degrade crystalline cellulose. No genes encoding proteins specialized in loosening or disruption the crystalline structure of cellulose were… read more here.
Keywords: hutchinsonii; crystalline cellulose; degradation; chu ... See more keywords