Articles with "culture dependent" as a keyword



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Accessing the Hidden Microbial Diversity of Aphids: an Illustration of How Culture-Dependent Methods Can Be Used to Decipher the Insect Microbiota

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Published in 2017 at "Microbial Ecology"

DOI: 10.1007/s00248-017-1092-x

Abstract: Microorganism communities that live inside insects can play critical roles in host development, nutrition, immunity, physiology, and behavior. Over the past decade, high-throughput sequencing reveals the extraordinary microbial diversity associated with various insect species and… read more here.

Keywords: insects; diversity; culture dependent; microbial diversity ... See more keywords
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Direct comparison of culture-dependent and culture-independent molecular approaches reveal the diversity of fungal endophytic communities in stems of grapevine (Vitis vinifera)

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Published in 2018 at "Fungal Diversity"

DOI: 10.1007/s13225-018-0399-3

Abstract: Grapevines (Vitis vinifera) are colonized by ubiquitous microorganisms known as endophytes, which may have advantageous or neutral effects without causing disease symptoms. Certain endophytes are uncultivable, so culture-independent approaches such as next generation sequencing (NGS)… read more here.

Keywords: culture dependent; culture; culture independent; vitis vinifera ... See more keywords
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Bacterial diversity analysis in Kazakh fermented milks Shubat and Ayran by combining culture-dependent and culture-independent methods

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Published in 2021 at "Lwt - Food Science and Technology"

DOI: 10.1016/j.lwt.2021.110877

Abstract: Abstract Shubat (fermented camel milk beverage) and Ayran (yogurt) are popular traditional fermented milk products in Kazakhstan. However, the natural microbial diversity in these fermented dairy products is not well studied. In this study, we… read more here.

Keywords: shubat; culture dependent; bacterial diversity; shubat ayran ... See more keywords
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Analysis of Phyllosphere Microorganisms and Potential Pathogens of Tobacco Leaves

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Published in 2022 at "Frontiers in Microbiology"

DOI: 10.3389/fmicb.2022.843389

Abstract: In the tobacco phyllosphere, some of the microbes may have detrimental effects on plant health, while many may be neutral or even beneficial. Some cannot be cultivated, so culture-independent methods are needed to explore microbial… read more here.

Keywords: culture dependent; symptomatic leaves; tobacco; analysis ... See more keywords
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Bacteria Associated With a Commercial Mycorrhizal Inoculum: Community Composition and Multifunctional Activity as Assessed by Illumina Sequencing and Culture-Dependent Tools

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Published in 2019 at "Frontiers in Plant Science"

DOI: 10.3389/fpls.2018.01956

Abstract: The implementation of sustainable agriculture encompasses practices enhancing the activity of beneficial soil microorganisms, able to modulate biogeochemical soil cycles and to affect soil fertility. Among them, arbuscular mycorrhizal fungi (AMF) establish symbioses with the… read more here.

Keywords: culture dependent; community; associated commercial; inoculum ... See more keywords
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Culture-Dependent and Culture-Independent Characterization of the Olive Xylem Microbiota: Effect of Sap Extraction Methods

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Published in 2019 at "Frontiers in Plant Science"

DOI: 10.3389/fpls.2019.01708

Abstract: Microbial endophytes are well known to protect host plants against pathogens, thus representing a promising strategy for the control of xylem-colonizing pathogens. To date, the vast majority of microbial communities inhabiting the olive xylem are… read more here.

Keywords: olive xylem; culture dependent; sap; effect ... See more keywords
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Culture-Dependent and Sequencing Methods Revealed the Absence of a Bacterial Community Residing in the Urine of Healthy Cats

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Published in 2020 at "Frontiers in Veterinary Science"

DOI: 10.3389/fvets.2020.00438

Abstract: A growing number of studies suggest that the lower urinary tract of humans and dogs can harbor a urinary microbiota. Nevertheless, a certain concern has developed that the microbiota reported could be due to unaccounted… read more here.

Keywords: healthy cats; culture dependent; bacterial community; culture ... See more keywords
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Culture-Dependent and Amplicon Sequencing Approaches Reveal Diversity and Distribution of Black Fungi in Antarctic Cryptoendolithic Communities

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Published in 2021 at "Journal of Fungi"

DOI: 10.3390/jof7030213

Abstract: In the harshest environmental conditions of the Antarctic desert, normally incompatible with active life, microbes are adapted to exploit the cryptoendolithic habitat (i.e., pore spaces of rocks) and represent the predominant life-forms. In the rocky… read more here.

Keywords: diversity; black fungi; culture dependent; diversity distribution ... See more keywords
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Hidden Fungi: Combining Culture-Dependent and -Independent DNA Barcoding Reveals Inter-Plant Variation in Species Richness of Endophytic Root Fungi in Elymus repens

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Published in 2021 at "Journal of Fungi"

DOI: 10.3390/jof7060466

Abstract: The root endophyte community of the grass species Elymus repens was investigated using both a culture-dependent approach and a direct amplicon sequencing method across five sites and from individual plants. There was much heterogeneity across… read more here.

Keywords: culture dependent; root; species richness; elymus repens ... See more keywords
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The Existence of Endophytic Actinobacteria from Rhododendron zoelerri Revealed by Culture-Dependent and Culture-Independent Approaches

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Published in 2018 at "HAYATI Journal of Biosciences"

DOI: 10.4308/hjb.25.2.54

Abstract: Endophytic actinobacteria from medicinal plant may play a significant role in producing bioactive compounds. The information regarding their diversity is an important.  Rhododendron are traditionally used for treating human disorders. One of the selected Rhododendron… read more here.

Keywords: zoelleri; culture independent; culture dependent; existence endophytic ... See more keywords