Assessing the potential of culture-independent 16S rRNA microbiome analysis in disease diagnostics: the example of Dianthus gratianopolitanus and Robbsia andropogonis
Sign Up to like & getrecommendations! Published in 2019 at "European Journal of Plant Pathology"
DOI: 10.1007/s10658-019-01850-8
Abstract: The goal of this study was to determine if culture-independent 16S rRNA sequencing of plant-associated microbiomes could facilitate disease diagnosis of cheddar pinks (Dianthus gratianopolitanus) with symptoms of leaf spotting at a Virginia nursery. The… read more here.
Keywords: culture independent; disease; 16s rrna; andropogonis ... See more keywords
Direct comparison of culture-dependent and culture-independent molecular approaches reveal the diversity of fungal endophytic communities in stems of grapevine (Vitis vinifera)
Sign Up to like & getrecommendations! Published in 2018 at "Fungal Diversity"
DOI: 10.1007/s13225-018-0399-3
Abstract: Grapevines (Vitis vinifera) are colonized by ubiquitous microorganisms known as endophytes, which may have advantageous or neutral effects without causing disease symptoms. Certain endophytes are uncultivable, so culture-independent approaches such as next generation sequencing (NGS)… read more here.
Keywords: culture dependent; culture; culture independent; vitis vinifera ... See more keywords
Culture-independent multilocus sequence typing of Pseudomonas aeruginosa for cross-infection screening.
Sign Up to like & getrecommendations! Published in 2021 at "Diagnostic microbiology and infectious disease"
DOI: 10.1016/j.diagmicrobio.2021.115315
Abstract: The genotyping of pathogens within cystic fibrosis cohorts is an important process, enabling the detection of transmissible and clinically-important strains. Traditionally this has been via culture-dependent processes. However, culture-independent investigation of respiratory samples is becoming… read more here.
Keywords: pseudomonas aeruginosa; culture independent; culture; sequence typing ... See more keywords
Culture‐independent Characterization of the Microbiome of Healthy Pulp
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Endodontics"
DOI: 10.1016/j.joen.2018.03.009
Abstract: Introduction: Advances in culture‐independent molecular biotechnologies have driven a greater appreciation for the function of mutualistic microorganisms in the maintenance of states of health in humans. The purpose of this study was to test the… read more here.
Keywords: bacterial dna; culture independent; dna; healthy pulp ... See more keywords
Culture Independent Diversity of Bacterial Communities Indigenous to Lower Altitude at Laohugou Glacial Environment
Sign Up to like & getrecommendations! Published in 2020 at "Geomicrobiology Journal"
DOI: 10.1080/01490451.2020.1797946
Abstract: Abstract High-throughput sequencing approach of the 16S rRNA gene was employed to evaluate the bacterial diversity inhabit in melted water, snow, soil, and rocks samples at the lower altitudes of the Laohugou glacial environment. Bioinformatics… read more here.
Keywords: culture independent; glacial environment; laohugou glacial; diversity ... See more keywords
Microbiome Profiles of Nebulizers in Hospital Use.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of aerosol medicine and pulmonary drug delivery"
DOI: 10.1089/jamp.2021.0032
Abstract: Background: Nebulizers are used to provide treatment to respiratory patients. Concerns over nosocomial infection risks from contaminated nebulizers raise the critical need to identify all microbial populations in nebulizers used by patients. However, conventional culture-dependent… read more here.
Keywords: culture; used patients; nebulizers used; microbial populations ... See more keywords
One clone to rule them all: Culture-independent genomics of Chlamydia psittaci from equine and avian hosts in Australia
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Genomics"
DOI: 10.1099/mgen.0.000888
Abstract: Chlamydia psittaci is an avian pathogen with zoonotic potential. In Australia, C. psittaci has been well reported as a cause of reproductive loss in mares which subsequently have been the source of infection and illness… read more here.
Keywords: chlamydia psittaci; equine; snp; culture independent ... See more keywords
Culture-independent tracking of Vibrio cholerae lineages reveals complex spatiotemporal dynamics in a natural population.
Sign Up to like & getrecommendations! Published in 2020 at "Environmental microbiology"
DOI: 10.1111/1462-2920.14921
Abstract: Populations of the bacterium Vibrio cholerae consist of dozens of distinct lineages, with primarily (but not exclusively) members of the pandemic generating (PG) lineage capable of causing the diarrheal disease cholera. Assessing composition and temporal… read more here.
Keywords: vibrio cholerae; cholerae; culture independent; cholerae lineages ... See more keywords
Culture-Independent Detection and Identification of Leptospira Serovars
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.02475-22
Abstract: Leptospirosis is caused by a diverse group of pathogenic spirochetes comprising over 200 different serotypes. Some are widely reported and of public health importance owing to their propensity to produce severe, fatal disease in humans.… read more here.
Keywords: culture; leptospira; culture independent; group pathogenic ... See more keywords
Culture-Independent Analyses Reveal Novel Anaerolineaceae as Abundant Primary Fermenters in Anaerobic Digesters Treating Waste Activated Sludge
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2017.01134
Abstract: Anaerobic digestion for biogas production is reliant on the tightly coupled synergistic activities of complex microbial consortia. Members of the uncultured A6 phylotype, within the phylum Chloroflexi, are among the most abundant genus-level-taxa of mesophilic… read more here.
Keywords: culture independent; primary fermenters; analyses reveal; novel anaerolineaceae ... See more keywords
An Improved Real-Time Viability PCR Assay to Detect Salmonella in a Culture-Independent Era
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232314708
Abstract: Viability PCR (vPCR) uses a DNA intercalating dye to irreversibly bind double-stranded DNA from organisms with compromised cell membranes. This allows the selective amplification of DNA from intact cells. An optimized vPCR protocol should minimize… read more here.
Keywords: viability pcr; cell; culture independent; dna ... See more keywords