Flow cytometric resolution of yeast is affected by enzymatic treatment and culture media
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13456
Abstract: Saccharomyces cerevisiae is an important model organism and a typical fungal representative for studies of eukaryotes. The cell cycle of yeast can be analyzed by flow cytometry, and refining the cytometric resolution of the cell… read more here.
Keywords: enzymatic treatment; cytometric resolution; resolution; culture media ... See more keywords
Vitamins in cell culture media: Stability and stabilization strategies
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26942
Abstract: Nowadays, chemically defined cell culture media (CCM) have replaced serum‐ and hydrolysate‐based media that rely on complex ingredients, such as yeast extracts or peptones. Benefits include a significantly lower lot‐to‐lot variability, more efficient manufacturing by… read more here.
Keywords: stabilization; cell culture; culture media; vitamins cell ... See more keywords
Multi‐information source Bayesian optimization of culture media for cellular agriculture
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28132
Abstract: Culture media used in industrial bioprocessing and the emerging field of cellular agriculture is difficult to optimize due to the lack of rigorous mathematical models of cell growth and culture conditions, as well as the… read more here.
Keywords: culture; multi information; information source; culture media ... See more keywords
Resveratrol addition to Chinese hamster ovary cell culture media: The effect on cell growth, monoclonal antibody synthesis, and its chemical modification
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Progress"
DOI: 10.1002/btpr.2940
Abstract: The effect of the addition of resveratrol to cell culture media during the production of monoclonal antibodies was investigated. Treatments of Chinese hamster ovary (CHO) cells expressing immunoglobulin G (IgG) with 25 and 50 μM resveratrol… read more here.
Keywords: resveratrol; igg; cell culture; chinese hamster ... See more keywords
Rapid metal speciation of cell culture media using reversed‐phase separations and inductively coupled plasma optical emission spectrometry
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3311
Abstract: Cell culture media metal content is critical in mammalian cell growth and monoclonal antibody productivity. The variability in metal concentrations has multiple sources of origin. As such, there is a need to analyze media before,… read more here.
Keywords: cell culture; cell; inductively coupled; culture media ... See more keywords
Flaming as part of aseptic technique increases CO2 (g) and decreases pH in freshwater culture media
Sign Up to like & getrecommendations! Published in 2020 at "Limnology and Oceanography: Methods"
DOI: 10.1002/lom3.10355
Abstract: Aseptic technique has historically served as a fundamental practice in microbiology, helping to maintain culture purity and integrity. This technique has been widely encouraged and employed for use with cultures of heterotrophic bacteria as well… read more here.
Keywords: approaches may; culture; flaming part; aseptic technique ... See more keywords
Rapid isolation of lentivirus particles from cell culture media via a hydrophobic interaction chromatography method on a polyester, capillary-channeled polymer fiber stationary phase
Sign Up to like & getrecommendations! Published in 2021 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-021-03232-8
Abstract: Lentiviruses are increasingly used as gene delivery vehicles for vaccines and immunotherapies. However, the purification of clinical-grade lentivirus vectors for therapeutic use is still troublesome and limits preclinical and clinical experiments. Current purification methods such… read more here.
Keywords: chromatography; method; lentivirus; culture media ... See more keywords
Evaluating haloarchaeal culture media for ultrahigh-molecular-weight polyhydroxyalkanoate biosynthesis by Haloferax mediterranei.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11508-3
Abstract: A series of culture media for haloarchaea were evaluated to optimize the production of ultrahigh-molecular-weight (UHMW) poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by Haloferax mediterranei. Cells of H. mediterranei grew (> 1 g/L of dry cell weight) and accumulated PHBV upon… read more here.
Keywords: molecular weight; uhmw phbv; culture media; phbv ... See more keywords
Culture media based on effluent derived from soy protein concentrate production for Lacticaseibacillus paracasei 90 biomass production: statistical optimisation, mineral characterization, and metabolic activities.
Sign Up to like & getrecommendations! Published in 2021 at "Antonie van Leeuwenhoek"
DOI: 10.1007/s10482-021-01660-1
Abstract: The waste and by-products of the soybean industry could be an economic source of nutrients to satisfy the high nutritional demands for the cultivation of lactic acid bacteria. The aims of this work were to… read more here.
Keywords: lacticaseibacillus paracasei; production; effluent derived; biomass production ... See more keywords
Influencing in vitro clonal propagation of Chonemorpha fragrans (moon) Alston by culture media strength, plant growth regulators, carbon source and photo periodic incubation
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Forestry Research"
DOI: 10.1007/s11676-018-0794-3
Abstract: Chonemorpha fragrans is an endangered medicinal woody climber, regarded among alternative plant sources of camptothecin. Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S. dollars in the recent, and is… read more here.
Keywords: strength; growth regulators; plant; plant growth ... See more keywords
Comparative Analysis of MSC-Derived Exosomes Depending on Cell Culture Media for Regenerative Bioactivity.
Sign Up to like & getrecommendations! Published in 2021 at "Tissue engineering and regenerative medicine"
DOI: 10.1007/s13770-021-00352-1
Abstract: BACKGROUND In order to produce and isolate the exosome derived from the cell of interests, a serum free environment (starvation) has been essential for excluding the unknown effect from serum-derived exosomes. Recently, serum-free culture media… read more here.
Keywords: cell culture; serum free; culture media; cell ... See more keywords