Development of a bead‐based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry
Sign Up to like & getrecommendations! Published in 2017 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23170
Abstract: Quantifying cytokines is extremely important in studies of host–pathogen interactions. Multiplex assays are commercially available but only for human and mouse cytokines. Here a method for the simultaneous quantification of five important bovine cytokines IFNγ,… read more here.
Keywords: culture supernatants; bovine cytokines; simultaneous quantification; flow cytometry ... See more keywords
Use of bacterial culture supernatants as anti-biofilm agents against Pseudomonas aeruginosa and Klebsiella pneumoniae.
Sign Up to like & getrecommendations! Published in 2022 at "European review for medical and pharmacological sciences"
DOI: 10.26355/eurrev_202202_28132
Abstract: OBJECTIVE Pseudomonas aeruginosa and Klebsiella pneumoniae are the most pervasive and challenging agents of bacterial nosocomial infections. Previous studies indicated that the microbial biofilms formed by these bacteria may play important roles in their pathogenesis… read more here.
Keywords: culture; culture supernatants; pneumoniae; biofilm ... See more keywords
A Straightforward Assay for Screening and Quantification of Biosurfactants in Microbial Culture Supernatants
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2020.00958
Abstract: A large variety of microorganisms produces biosurfactants with the potential for a number of diverse industrial applications. To identify suitable wild-type or engineered production strains, efficient screening methods are needed, allowing for rapid and reliable… read more here.
Keywords: straightforward assay; culture supernatants; quantification biosurfactants; assay screening ... See more keywords
Corynebacterium accolens inhibits Staphylococcus aureus induced mucosal barrier disruption
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.984741
Abstract: Background Corynebacterium accolens (C. accolens) is a common nasal colonizer, whereas Staphylococcus aureus (S. aureus) is typically regarded a pathogenic organism in patients with chronic rhinosinusitis (CRS). This study aims to evaluate the interaction of… read more here.
Keywords: mucosal barrier; culture supernatants; corynebacterium accolens; cell free ... See more keywords
Inactivation of Ebola Virus and SARS-CoV-2 in Cell Culture Supernatants and Cell Pellets by Gamma Irradiation
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v15010043
Abstract: Viral pathogens with the potential to cause widespread disruption to human health and society continue to emerge or re-emerge around the world. Research on such viruses often involves high biocontainment laboratories (BSL3 or BSL4), but… read more here.
Keywords: culture supernatants; gamma irradiation; supernatants cell; cell pellets ... See more keywords