MicroRNA In Situ Hybridization in Paraffin-Embedded Cultured Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0623-0_6
Abstract: MicroRNA-21 (miR-21) is one of the most abundant microRNAs in cancer tissues and is considered a strong prognostic biomarker. In situ hybridization (ISH) analyses using locked nucleic acid (LNA) probes have shown that miR-21 is… read more here.
Keywords: cultured cells; paraffin embedded; hybridization; cancer ... See more keywords
Analysis of Plasmalogen Synthesis in Cultured Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6937-1_6
Abstract: Plasmalogen synthesis can be analyzed by metabolic labeling, followed by the separation of ethanolamine plasmalogens from glycerophospholipids on one-dimensional thin-layer chromatography. The vinyl-ether bond of plasmalogens is acid-labile, which allows separating plasmalogens as 2-acyl-glycerophospholipids from… read more here.
Keywords: analysis plasmalogen; cultured cells; plasmalogen synthesis; synthesis cultured ... See more keywords
Mechanical Deformation of Cultured Cells with Hydrogels.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7113-8_17
Abstract: Polyacrylamide hydrogels can be used to culture cells in a range of stiffness that can closer mimic physiological environments. Changes in environmental stiffness have been documented in conditions such as fibrosis, cancer, and aging. In… read more here.
Keywords: cultured cells; cells hydrogels; biology; deformation cultured ... See more keywords
Biomimetic Extracellular Matrices and Scaffolds Prepared from Cultured Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-13-0950-2_24
Abstract: Extracellular matrix (ECM) interacts with cells and provides important signals to control cell functions and to maintain homeostasis of living organisms. Composition of ECM in each tissue is dependent on cell type and cell phenotype.… read more here.
Keywords: cultured cells; ecm substrates; scaffolds prepared; substrates scaffolds ... See more keywords
Functional analysis of McSnRK1 (SNF1-related protein kinase 1) in regulating Na/K homeostasis in transgenic cultured cells and roots of halophyte Mesembryanthemum crystallinum
Sign Up to like & getrecommendations! Published in 2019 at "Plant Cell Reports"
DOI: 10.1007/s00299-019-02412-2
Abstract: Key messageTransgenic callus and roots of ice plant with altered SnRK1 function were established using Agrobacterium-mediated transformation. The role of McSnRK1 in controlling Na+ influx and Na/K ratio was demonstrated.AbstractSnRK1 kinases (SNF1-related protein kinase1) control… read more here.
Keywords: cultured cells; mcsnrk1; plant; ice plant ... See more keywords
Streamlined duplex live‐dead microplate assay for cultured cells
Sign Up to like & getrecommendations! Published in 2017 at "Experimental Eye Research"
DOI: 10.1016/j.exer.2017.05.011
Abstract: ABSTRACT A duplex fluorescence assay to assess the viability of cells cultured in multi‐well plates is described, which can be carried out in the original culture plate using a plate reader, without exchanges of culture… read more here.
Keywords: live dead; cultured cells; fluorescence; assay ... See more keywords
Short-term temperature change detections and frequency signals in single cultured cells using a microfabricated thermistor
Sign Up to like & getrecommendations! Published in 2020 at "Sensing and bio-sensing research"
DOI: 10.1016/j.sbsr.2019.100309
Abstract: Abstract Thermal detection is an effective means of investigating the thermodynamics, metabolic mechanisms, and functions of cells. Here, a sensing device for performing thermal measurements in single cultured cells is fabricated, and its characteristics are… read more here.
Keywords: cultured cells; temperature; short term; single cultured ... See more keywords
Simultaneous detection of the subcellular localization of RNAs and proteins in cultured cells by combined multicolor RNA-FISH and IF.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2016.09.010
Abstract: Fluorescence in situ hybridization (FISH) and immunofluorescence (IF) are sensitive techniques used for detecting nucleic acids and proteins in cultured cells. However, these techniques are rarely applied together, and standard protocols are not readily compatible… read more here.
Keywords: cultured cells; proteins cultured; rna fish; multicolor rna ... See more keywords
Enhancing Comprehensive Analysis of Secreted Glycoproteins from Cultured Cells without Serum Starvation.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c05126
Abstract: Glycoproteins secreted by cells play essential roles in the regulation of extracellular activities. Secreted glycoproteins are often reflective of cellular status, and thus glycoproteins from easily accessible bodily fluids can serve as excellent biomarkers for… read more here.
Keywords: cultured cells; analysis; without serum; serum starvation ... See more keywords
Fermentation Supernatant of Elderly Feces with Inulin and Partially Hydrolyzed Guar Gum Maintains the Barrier of Inflammation-Induced Caco-2/HT29-MTX-E12 Co-Cultured Cells
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Agricultural and Food Chemistry"
DOI: 10.1021/acs.jafc.2c06232
Abstract: Intestinal barrier function declines with aging. We evaluated the effect of dietary fibers and indigestible oligosaccharides on intestinal barrier function by altering the microbiota of the elderly. The feces were anaerobically cultured with indigestible dextrin,… read more here.
Keywords: cultured cells; elderly feces; intestinal barrier; inulin partially ... See more keywords
A Rapid Array-Based Approach to N-Glycan Profiling of Cultured Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.9b00303
Abstract: Typically, N-glycosylation studies done on cultured cells require up to millions of cells followed by lengthy preparation to release, isolate, and profile N-glycans. To overcome these limitations, we report a rapid array-based workflow for profiling… read more here.
Keywords: profiling; glycan profiling; cultured cells; approach glycan ... See more keywords