Manipulating cultured mammalian cells for mitosis research.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2020.02.001
Abstract: The study of mitosis has always relied on bulk-preparation biochemistry techniques (Mazia & Dan, 1952), but very early on lent itself to living, single cell microscopic techniques (Inoue, 1953; Taylor, 1959). Here we describe several… read more here.
Keywords: cultured mammalian; mitosis research; cells mitosis; mammalian cells ... See more keywords
Adaptation and Changes in Actin Dynamics and Cell Motility as Early Responses of Cultured Mammalian Cells to Altered Gravitational Vector
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DOI: 10.3390/ijms23116127
Abstract: Cultured mammalian cells have been shown to respond to microgravity (μG), but the molecular mechanism is still unknown. The study we report here is focused on molecular and cellular events that occur within a short… read more here.
Keywords: gravity; cultured mammalian; early responses; mammalian cells ... See more keywords
Reduced Toxicity of Trichothecenes, Isotrichodermol, and Deoxynivalenol, by Transgenic Expression of the Tri101 3-O-Acetyltransferase Gene in Cultured Mammalian FM3A Cells
Sign Up to like & getrecommendations! Published in 2019 at "Toxins"
DOI: 10.3390/toxins11110654
Abstract: In trichothecene-producing fusaria, isotrichodermol (ITDol) is the first intermediate with a trichothecene skeleton. In the biosynthetic pathway of trichothecene, a 3-O-acetyltransferase, encoded by Tri101, acetylates ITDol to a less-toxic intermediate, isotrichodermin (ITD). Although trichothecene resistance… read more here.
Keywords: acetyltransferase; cultured mammalian; fm3a; expression tri101 ... See more keywords