A biophotonic approach to measure pH in small volumes in vitro: quantifiable differences in metabolic flux around the cumulus-oocyte-complex (COC).
Sign Up to like & getrecommendations! Published in 2019 at "Journal of biophotonics"
DOI: 10.1002/jbio.201960038
Abstract: Unfertilised eggs (oocytes) release chemical biomarkers into the medium surrounding them. This provides an opportunity to monitor cell health and development during assisted reproductive processes if detected in a non-invasive manner. Here we report the… read more here.
Keywords: coc; biophotonic approach; approach measure; oocyte ... See more keywords
How in vitro maturation changes the proteome of ovine cumulus–oocyte complexes?
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23638
Abstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus–oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC‐MS/MS. Differences in protein abundances (p read more here.
Keywords: mature cocs; cumulus oocyte; vitro maturation; oocyte complexes ... See more keywords
Cumulus‐oocyte complexes from sows show differences in lipid metabolism compared to cumulus‐oocyte complexes from prepubertal gilts during in vitro maturation
Sign Up to like & getrecommendations! Published in 2023 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23685
Abstract: This study aimed to evaluate the effects of donor age on lipid metabolism during in vitro maturation (IVM) of pigs cumulus‐oocyte complexes (COCs). We evaluated transcript levels of genes, the percentage of ooplasm occupied by… read more here.
Keywords: maturation; cumulus oocyte; lipid metabolism; oocyte complexes ... See more keywords
Stromal-derived factor 1 directly promotes genes expressed within the ovulatory cascade in feline cumulus oocyte complexes
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Assisted Reproduction and Genetics"
DOI: 10.1007/s10815-018-1150-4
Abstract: PurposeWe hypothesized that the chemokine SDF1/CXCR4 system was present in feline cumulus-oocyte complexes (COCs) and that COCs cultured with SDF1 would directly upregulate gene expression in the ovulatory cascade.MethodsOvaries (n = 50) were obtained from adult domestic… read more here.
Keywords: feline cumulus; cumulus oocyte; feline; cocs ... See more keywords
Supplementing in vitro embryo production media by NPPC and sildenafil affect the cytoplasmic lipid content and gene expression of bovine cumulus-oocyte complexes and embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Reproductive biology"
DOI: 10.1016/j.repbio.2018.01.004
Abstract: In our study, we added natriuretic peptide type C (NPPC) and/or sildenafil during in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COCs) followed by in vitro culture (IVC) of embryos with or without sildenafil. We… read more here.
Keywords: cumulus; cumulus oocyte; embryo; nppc sildenafil ... See more keywords
L-arginine affects the IVM of cattle cumulus-oocyte complexes.
Sign Up to like & getrecommendations! Published in 2017 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2016.09.017
Abstract: Nitric oxide (NO) is identified as a signaling molecule involved in many cellular or physiological functions, including meiotic maturation of cattle oocytes. This study aimed to evaluate the effect of supplementation of culture medium with… read more here.
Keywords: control; arg; cumulus oocyte; cultivation ... See more keywords
Cumulus-oocyte interactions and programmed cell death in bovine embryos produced in vitro.
Sign Up to like & getrecommendations! Published in 2019 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2018.11.028
Abstract: This study investigated the interactions between cumulus cells (CCs) and oocytes and programmed cell death in bovine cumulus-oocyte complexes (COCs) with different morphological characteristics. DNA fragmentation was assessed in CCs at 0 and 24 h of… read more here.
Keywords: cumulus; cumulus oocyte; programmed cell; cell death ... See more keywords
Melatonin enhances in vitro developmental competence of cumulus-oocyte complexes collected by ovum pick-up in prepubertal and adult dairy cattle.
Sign Up to like & getrecommendations! Published in 2020 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2020.12.011
Abstract: Bovine oocytes from prepubertal donors have been used for in vitro embryo production to decrease the generation interval. However, reduced cumulus-oocyte competence, mainly attributed to increased apoptosis, has been observed in oocytes/embryos collected from prepubertal donors.… read more here.
Keywords: competence; adult; prepubertal adult; adult dairy ... See more keywords
An improved IVM method for cumulus-oocyte complexes from small follicles in polycystic ovary syndrome patients enhances oocyte competence and embryo yield
Sign Up to like & getrecommendations! Published in 2017 at "Human Reproduction"
DOI: 10.1093/humrep/dex262
Abstract: STUDY QUESTION Are meiotic and developmental competence of human oocytes from small (2-8 mm) antral follicles improved by applying an optimized IVM method involving a prematuration step in presence of C-Type Natriuretic Peptide (CNP) followed… read more here.
Keywords: method; pmc; study; cumulus oocyte ... See more keywords
Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep
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DOI: 10.1371/journal.pone.0229043
Abstract: Oocyte in vitro maturation can be improved by mimicking the intra-follicular environment. Oocyte, cumulus cells, granulosa cells, and circulating factors act as meiotic regulators in follicles and maintain oocyte in the meiotic phase until oocyte… read more here.
Keywords: cumulus; oocyte; cumulus oocyte; oocyte complexes ... See more keywords
One-step automated bioprinting-based method for cumulus-oocyte complex microencapsulation for 3D in vitro maturation
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DOI: 10.1371/journal.pone.0238812
Abstract: Three-dimensional in vitro maturation (3D IVM) is a promising approach to improve IVM efficiency as it could prevent cumulus-oocyte complex (COC) flattening and preserve its structural and functional integrity. Methods reported to date have low… read more here.
Keywords: bioprinting based; cumulus oocyte; vitro maturation; maturation ... See more keywords