Alka(e)ne synthesis in Cupriavidus necator boosted by the expression of endogenous and heterologous ferredoxin–ferredoxin reductase systems
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26805
Abstract: To boost aldehyde deformylating oxygenase (ADO) activity in a Cupriavidus necator strain expressing a synthetic alkane pathway, the expression of two ferredoxin–ferredoxin reductase systems was tested. The genes of a native fd/FNR‐like system were identified… read more here.
Keywords: necator; ferredoxin; production; ferredoxin ferredoxin ... See more keywords
Polyhydroxyalkanoate production from sucrose by Cupriavidus necator strains harboring csc genes from Escherichia coli W
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8470-7
Abstract: Cupriavidus necator H16 is the most promising bacterium for industrial production of polyhydroxyalkanoates (PHAs) because of their remarkable ability to accumulate them in the cells. With genetic modifications, this bacterium can produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx), which… read more here.
Keywords: genes escherichia; necator; csc genes; production ... See more keywords
Chemoautotroph Cupriavidus necator as a potential game-changer for global warming and plastic waste problem: A review.
Sign Up to like & getrecommendations! Published in 2021 at "Bioresource technology"
DOI: 10.1016/j.biortech.2021.125693
Abstract: Cupriavidus necator, a versatile microorganism found in both soil and water, can have both heterotrophic and lithoautotrophic metabolisms depending on environmental conditions. C. necator has been extensively examined for producing Polyhydroxyalkanoates (PHAs), the promising polyester… read more here.
Keywords: necator; waste; necator potential; cupriavidus necator ... See more keywords
The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Microbiological Methods"
DOI: 10.1016/j.mimet.2021.106323
Abstract: Cupriavidus necator H16 can convert CO2 into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application… read more here.
Keywords: cupriavidus; necator h16; pmtl70000 modular; cupriavidus necator ... See more keywords
Heterologous expression of phaC2 gene and poly-3-hydroxyalkanoate production by recombinant Cupriavidus necator strains using canola oil as carbon source.
Sign Up to like & getrecommendations! Published in 2018 at "New biotechnology"
DOI: 10.1016/j.nbt.2017.08.001
Abstract: Many heterologous transformation studies have been carried out using the Cupriavidus necator PHB-4 strain to investigate the expression characteristics of various polyhydroxyalkanoate (PHA) synthase enzymes. In this study, we generated a recombinant C. necator PHB-4… read more here.
Keywords: necator; phac2 gene; expression; gene ... See more keywords
pCAT vectors overcome inefficient electroporation of Cupriavidus necator H16.
Sign Up to like & getrecommendations! Published in 2021 at "New biotechnology"
DOI: 10.1016/j.nbt.2021.07.003
Abstract: Cupriavidus necator H16 is a chemolithoautotroph with a range of industrial biotechnological applications. Advanced metabolic engineering in the bacterium, however, is impeded by low transformation efficiency, making it difficult to introduce and screen new genetic… read more here.
Keywords: necator h16; cupriavidus necator; pcat vectors;
Purification and characterisation of alliinase produced by Cupriavidus necator and its application for generation of cytotoxic agent: Allicin
Sign Up to like & getrecommendations! Published in 2018 at "Saudi Journal of Biological Sciences"
DOI: 10.1016/j.sjbs.2016.01.003
Abstract: Allicin, an extremely active constituent of freshly crushed garlic, is produced upon reaction of alliin with the enzyme alliinase (EC 4.4.1.4). A bacterium Cupriavidus necator with the ability of alliinase production was isolated from a… read more here.
Keywords: alliinase; agent allicin; purification; cupriavidus necator ... See more keywords
Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production.
Sign Up to like & getrecommendations! Published in 2017 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2017.05.007
Abstract: We previously reported a metabolic engineering strategy to develop an isopropanol producing strain of Cupriavidus necator leading to production of 3.4gL-1 isopropanol. In order to reach higher titers, isopropanol toxicity to the cells has to… read more here.
Keywords: isopropanol; cupriavidus necator; production; groesl ... See more keywords
Utilization of Dielectrophoresis for the Quantification of Rare Earth Elements Adsorbed on Cupriavidus necator
Sign Up to like & getrecommendations! Published in 2019 at "ACS Sustainable Chemistry & Engineering"
DOI: 10.1021/acssuschemeng.9b03878
Abstract: A new method of quantifying rare earth elements (REEs): light REEs (neodymium—Nd3+ and samarium—Sm3+) and heavy REE (europium—Eu3+) was investigated by utilizing native Cupriavidus necator as the biosorbent and dielectrophoresis as the quantification technique.C. necator… read more here.
Keywords: necator; earth elements; dielectrophoresis quantification; rare earth ... See more keywords
Identification and characterization of L- and D-lactate-inducible systems from Escherichia coli MG1655, Cupriavidus necator H16 and Pseudomonas species
Sign Up to like & getrecommendations! Published in 2022 at "Scientific Reports"
DOI: 10.1038/s41598-022-06028-7
Abstract: Lactic acid is an important platform chemical used for the production of various compounds including polylactic acid (PLA). Optically pure L- and D-lactic acids are required to obtain high quality PLA. To advance the development… read more here.
Keywords: systems escherichia; cupriavidus necator; escherichia coli; lactate inducible ... See more keywords
Minimizing the Lag Phase of Cupriavidus necator Growth under Autotrophic, Heterotrophic, and Mixotrophic Conditions
Sign Up to like & getrecommendations! Published in 2023 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.02007-22
Abstract: The current work investigated the inhibition of organic and gaseous substrates on the microbial adaption of Cupriavidus necator under several metabolic conditions commonly employed for commercial polyhydroxyalkanoate production. We also proposed a two-stage cultivation system… read more here.
Keywords: lag phase; cupriavidus necator; growth; phase ... See more keywords