Imaging Precision-Cut Lung Slices to Visualize Leukocyte Localization and Trafficking.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7896-0_18
Abstract: Pulmonary dendritic cells (DCs) are potent antigen-presenting cells that can activate both naïve and memory/effector T cells. However, very little is known of how movements and localization of DCs contribute to these events. To study… read more here.
Keywords: cut lung; precision cut; lung slices; localization ... See more keywords
Precision cut lung slices: an ex vivo model for assessing the impact of immunomodulatory therapeutics on lung immune responses.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of toxicology"
DOI: 10.1007/s00204-021-03096-y
Abstract: Chronic inflammatory diseases of the respiratory tract, such as chronic obstructive pulmonary disease (COPD) and asthma, are severe lung diseases that require effective treatments. In search for new medicines for these diseases, there is an… read more here.
Keywords: cut lung; precision cut; vivo model; lung slices ... See more keywords
Small RNA-based formulations in Precision cut lung slices - is there an alternative for in vivo/in vitro studies?
Sign Up to like & getrecommendations! Published in 2025 at "Expert review of respiratory medicine"
DOI: 10.1080/17476348.2025.2600109
Abstract: INTRODUCTION Precision cut lung slices (PCLS) are ex vivo models to study lung diseases. They have numerous advantages related to the presence of different cell types and extracellular matrix. They are an excellent platform to… read more here.
Keywords: lung slices; based formulations; rna based; small rna ... See more keywords
Cryopreserved human precision-cut lung slices provide an immune competent pulmonary test system for “on-demand” use and long-term cultures
Sign Up to like & getrecommendations! Published in 2023 at "Toxicological Sciences"
DOI: 10.1093/toxsci/kfac136
Abstract: Abstract Human precision-cut lung slices (hPCLS), considered a highly relevant ex vivo model of the lung, offer native architecture and cells of the lung tissue including respiratory parenchyma, small airways, and immune competent cells. However,… read more here.
Keywords: cut lung; lung slices; lung; human precision ... See more keywords
Precision-cut lung slices: a powerful ex vivo model to investigate respiratory infectious diseases.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular microbiology"
DOI: 10.1111/mmi.14817
Abstract: Respiratory infections are leading causes of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid or surrogate animal models. Although these provide important insights, they have limitations.… read more here.
Keywords: cut lung; precision cut; lung slices; respiratory ... See more keywords
Multiscale stiffness of human emphysematous precision cut lung slices
Sign Up to like & getrecommendations! Published in 2023 at "Science Advances"
DOI: 10.1126/sciadv.adf2535
Abstract: Emphysema is a debilitating disease that remodels the lung leading to reduced tissue stiffness. Thus, understanding emphysema progression requires assessing lung stiffness at both the tissue and alveolar scales. Here, we introduce an approach to… read more here.
Keywords: precision cut; cut lung; stiffness; lung ... See more keywords
Optimizing miRNA transfection for screening in precision cut lung slices
Sign Up to like & getrecommendations! Published in 2024 at "American Journal of Physiology - Lung Cellular and Molecular Physiology"
DOI: 10.1152/ajplung.00138.2024
Abstract: Precision cut lung slices(PCLS) are complex 3D lung tissue models, which preserve the native microenvironment, including cell diversity and cell-matrix interactions. They are an innovative ex vivo platform that allows studying disease as well as… read more here.
Keywords: lung slices; lung; physiology; transfection ... See more keywords
Early Cytokine Induction Upon Pseudomonas aeruginosa Infection in Murine Precision Cut Lung Slices Depends on Sensing of Bacterial Viability
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2020.598636
Abstract: Breathing allows a multitude of airborne microbes and microbial compounds to access the lung. Constant exposure of the pulmonary microenvironment to immunogenic particles illustrates the need for proper control mechanisms ensuring the differentiation between threatening… read more here.
Keywords: cut lung; viability; precision cut; infection ... See more keywords
Perspectives on precision cut lung slices—powerful tools for investigation of mechanisms and therapeutic targets in lung diseases
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Pharmacology"
DOI: 10.3389/fphar.2023.1162889
Abstract: Precision cut lung slices (PCLS) have emerged as powerful experimental tools for respiratory research. Pioneering studies using mouse PCLS to visualize intrapulmonary airway contractility have been extended to pulmonary arteries and for assessment of novel… read more here.
Keywords: mechanisms therapeutic; precision cut; cut lung; lung ... See more keywords
A Virulent Trueperella pyogenes Isolate, Which Causes Severe Bronchoconstriction in Porcine Precision-Cut Lung Slices
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2021.824349
Abstract: Trueperella pyogenes causes disease in cattle, sheep, goats and swine, and is involved occasionally in human disease worldwide. Most reports implicating T. pyogenes have been associated with clinical cases, whereas no report has focused on… read more here.
Keywords: lung; bronchoconstriction; cut lung; precision cut ... See more keywords