A tissue dissociation method for ATAC-seq and CUT&RUN in Drosophila pupal tissues
Sign Up to like & getrecommendations! Published in 2023 at "Fly"
DOI: 10.1080/19336934.2023.2209481
Abstract: ABSTRACT Chromatin accessibility, histone modifications, and transcription factor binding are highly dynamic during Drosophila metamorphosis and drive global changes in gene expression as larval tissues differentiate into adult structures. Unfortunately, the presence of pupa cuticle… read more here.
Keywords: histone modifications; dissociation method; seq; cut run ... See more keywords
Abstract 4718: Analyses of epigenetic marks and mechanisms in disease: Your guide to a successful CUT&RUN assay
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-4718
Abstract: Like the chromatin immunoprecipitation (ChIP) assay, Cleavage Under Targets & Release Using Nuclease (CUT&RUN) is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell. The CUT&RUN… read more here.
Keywords: analyses epigenetic; cut; cut run; marks mechanisms ... See more keywords
ssvQC: an integrated CUT&RUN quality control workflow for histone modifications and transcription factors
Sign Up to like & getrecommendations! Published in 2021 at "BMC Research Notes"
DOI: 10.1186/s13104-021-05781-8
Abstract: Objective Among the different methods to profile the genome-wide patterns of transcription factor binding and histone modifications in cells and tissues, CUT&RUN has emerged as a more efficient approach that allows for a higher signal-to-noise… read more here.
Keywords: quality; histone modifications; transcription; cut run ... See more keywords
Integrative Cistromic and Transcriptomic Analyses Identify CREB Target Genes in Cystic Renal Epithelial Cells.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the American Society of Nephrology : JASN"
DOI: 10.1681/asn.2021010101
Abstract: Background Genome-wide mapping of transcription factor (TF) binding sites is essential to identify a TF's direct target genes in kidney development and diseases. However, due to the cellular complexity of the kidney and limited numbers… read more here.
Keywords: cut run; epithelial cells; kidney; target genes ... See more keywords
Genome-wide profiling of histone modifications in Plasmodium falciparum using CUT&RUN
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DOI: 10.26508/lsa.202201778
Abstract: We present a detailed step-wise protocol for reliable genome-wide profiling of histone modifications using CUT&RUN that requires only low numbers of Plasmodium falciparum blood stages. We recently adapted a CUT&RUN protocol for genome-wide profiling of… read more here.
Keywords: histone modifications; cut run; genome wide; wide profiling ... See more keywords