Auto-cycling primer extension for amplified microRNA detection.
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DOI: 10.1039/d2cc01784a
Abstract: The current polymerase-based nucleic acid amplification techniques cannot maintain continuous polymerization reactions unless by changing the temperature or adding additional reagents (e.g. the second enzyme or betaine), which undoubtedly increases the cost and operation steps.… read more here.
Keywords: cycling primer; primer extension; detection; auto cycling ... See more keywords
Dual amplification of a bio-barcode and auto-cycling primer extension for highly sensitive detection of miRNA.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc03864a
Abstract: MicroRNAs (miRNAs) can be used as biomarkers for the diagnosis and therapy of cancers. However, their low abundance and the complex environment in biological samples hinder miRNA detection. A dual amplification strategy based on the… read more here.
Keywords: cycling primer; auto cycling; amplification; detection ... See more keywords