OR30. HLA expression on CD34+ stem cells: is a flow cytometric crossmatch a good gatekeeper for mismatched hematopoietic cell transplantion?
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DOI: 10.1016/j.humimm.2018.07.035
Abstract: Aim Primary graft failure associated with donor specific antibody (DSA) is influenced by the level of circulating DSA at time of infusion and by the density of HLA antigens expressed on cell populations needed for… read more here.
Keywords: download; cytometric crossmatch; flow cytometric; hla ... See more keywords
P127 Transfer of flow cytometric crossmatch data from the BD facscanto II into the histotrac database
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DOI: 10.1016/j.humimm.2018.07.184
Abstract: Aim Flow Cytometric Crossmatch (XM) is critical to the work-up of renal transplant patients. The complexity of the data acquired in XM testing and the amount of manual data entry into reporting software is a… read more here.
Keywords: utility; control; cytometric crossmatch; flow cytometric ... See more keywords
P137 Go pro(nase)! the importance of donor lymphocyte treatment with pronase in flow cytometric crossmatch testing
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DOI: 10.1016/j.humimm.2018.07.194
Abstract: Aim Flow cytometric crossmatch (FCXM) is performed to confirm donor/recipient histocompatibility. Previous multicenter study (Liwski et al. ASHI 2012) suggested that protocol differences contribute to FCXM result variability and that pronase treatment improves B cell… read more here.
Keywords: treatment; cytometric crossmatch; flow cytometric; donor ... See more keywords
P154 Variability in the sensitivity of the flow cytometric crossmatch
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DOI: 10.1016/j.humimm.2018.07.211
Abstract: Aim The sensitivity of the flow cytometric crossmatch (FXM) is often variable across labs in the presence of weak donor specific antibodies (DSA). In addition, inter-laboratory variation to the interpretation of Mean Fluorescence Intensity (MFI)… read more here.
Keywords: crossmatch; cytometric crossmatch; flow cytometric; sensitivity flow ... See more keywords