Cas9‐orthologue‐mediated cytosine and adenine base editors recognizing NNAAAA PAM sequences
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology Journal"
DOI: 10.1002/biot.202200533
Abstract: CRISPR/Cas9 system has been applied as an effective genome‐targeting technology. By fusing deaminases with Cas9 nickase (nCas9), various cytosine and adenine base editors (CBEs and ABEs) have been successfully developed that can efficiently induce nucleotide… read more here.
Keywords: cytosine adenine; pam sequences; base editors; adenine base ... See more keywords
Theoretical description of the preferential hydrolytic deamination of cytosine over adenine
Sign Up to like & getrecommendations! Published in 2021 at "Computational and Theoretical Chemistry"
DOI: 10.1016/j.comptc.2021.113354
Abstract: Abstract It has been shown experimentally that the hydrolytic deamination of cytosine in single-stranded DNA occurs significantly more frequently than that of adenine, despite similarities in their structure. To provide insight into this difference, we… read more here.
Keywords: deamination cytosine; hydrolytic deamination; deamination; cytosine adenine ... See more keywords
Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors
Sign Up to like & getrecommendations! Published in 2020 at "Nature Biotechnology"
DOI: 10.1038/s41587-019-0393-7
Abstract: Targeted saturation mutagenesis of crop genes could be applied to produce genetic variants with improved agronomic performance. However, tools for directed evolution of plant genes, such as error-prone PCR or DNA shuffling, are limited 1… read more here.
Keywords: mutagenesis; rice; base editors; plant genes ... See more keywords
Abstract A26: High frequency of Cytosine to Adenine mutations in neuroblastoma correlates with genomic aberrations in 8-Oxo-Guanine repair pathway
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DOI: 10.1158/1557-3125.dnarepair16-a26
Abstract: Background: Somatic mutations can be grouped into 96 tri-nucleotide patterns, termed mutational signatures. These signatures can provide insights into underlying processes that fuel the evolution of tumor cells, and may expose therapeutic intervention avenues. Here… read more here.
Keywords: ogg1 mutyh; frequency; cell lines; oxo guanine ... See more keywords
Estrogen Receptor-β Gene Cytosine-Adenine (ESR2-CA) Repeat Polymorphism in Postmenopausal Colon Cancer
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24054502
Abstract: The pathobiological role of estrogen is controversial in colorectal cancer. Cytosine-adenine (CA) repeat in the estrogen receptor (ER)-β gene (ESR2-CA) is a microsatellite, as well as representative of ESR2 polymorphism. Though its function is unknown,… read more here.
Keywords: cytosine adenine; expression; esr2; colon cancer ... See more keywords