New cytosine base editor for plant genome editing
Sign Up to like & getrecommendations! Published in 2018 at "Science China Life Sciences"
DOI: 10.1007/s11427-018-9416-y
Abstract: A large number of beneficial agronomic traits in crops are associated with single nucleotide polymorphisms (SNPs) or point mutations (Jiao et al., 2010; Li et al., 2017; Ma et al., 2015). In the past, site-specific… read more here.
Keywords: plant; hdr; pbe; cytosine base ... See more keywords
Highly efficient CRISPR-SaKKH tools for plant multiplex cytosine base editing
Sign Up to like & getrecommendations! Published in 2020 at "Crop Journal"
DOI: 10.1016/j.cj.2020.03.002
Abstract: Abstract Base editing, as an expanded clustered regularly interspaced short palindromic repeats (CRISPR)-Cas genome editing strategy, permits precise and irreversible nucleotide conversion. SaKKH, an efficient variant of a Cas9 ortholog from Staphylococcus aureus (SaCas9), is… read more here.
Keywords: base editing; sakkh; base; multiplex cytosine ... See more keywords
Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.07.005
Abstract: Cytosine base editors (CBEs) generate C-to-T nucleotide substitutions in genomic target sites without inducing double-strand breaks. However, CBEs such as BE3 can cause genome-wide off-target changes via sgRNA-independent DNA deamination. By leveraging the orthogonal R-loops… read more here.
Keywords: base editors; target; base; cytosine base ... See more keywords
Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-15887-5
Abstract: Cytosine base editors (CBEs) enable efficient, programmable reversion of T•A to C•G point mutations in the human genome. Recently, cytosine base editors with rAPOBEC1 were reported to induce unguided cytosine deamination in genomic DNA and cellular… read more here.
Keywords: dna; base editors; target; base ... See more keywords
BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-26461-y
Abstract: Adenine and cytosine base editors (ABE, CBE) allow for precision genome engineering. Here, Base Editor Activity Reporter (BEAR), a plasmid-based fluorescent tool is introduced, which can be applied to report on ABE and CBE editing… read more here.
Keywords: base editors; increased fidelity; base; cytosine base ... See more keywords
Precision genome engineering through adenine and cytosine base editing
Sign Up to like & getrecommendations! Published in 2018 at "Nature Plants"
DOI: 10.1038/s41477-018-0115-z
Abstract: Adenine base editors (ABEs), composed of an engineered deaminase and a catalytically impaired CRISPR–Cas9 variant, are powerful new tools for targeted base editing in cells and organisms. Together with cytosine base editors (CBEs), ABEs enable… read more here.
Keywords: base editing; engineering adenine; base; cytosine base ... See more keywords
Cytosine base editors go off-target
Sign Up to like & getrecommendations! Published in 2019 at "Nature Reviews Genetics"
DOI: 10.1038/s41576-019-0110-x
Abstract: Two studies in Science show that cytosine base editors, but not adenine base editors or CRISPR–Cas9, induce notable off-target single-nucleotide variants in rice and in mouse embryos. read more here.
Keywords: editors target; base editors; base; cytosine base ... See more keywords
A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects
Sign Up to like & getrecommendations! Published in 2020 at "Nature Methods"
DOI: 10.1038/s41592-020-0832-x
Abstract: Cytosine base editors (CBEs) offer a powerful tool for correcting point mutations, yet their DNA and RNA off-target activities have caused concerns in biomedical applications. We describe screens of 23 rationally engineered CBE variants, which… read more here.
Keywords: rna target; cytosine base; target; dna rna ... See more keywords
The efficacy of CRISPR-mediated cytosine base editing with the RPS5a promoter in Arabidopsis thaliana
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-87669-y
Abstract: CRISPR/Cas9-mediated genome editing is an important and versatile technology in modern biological research. Recent advancements include base-editing CRISPR tools that enable targeted nucleotide substitutions using a fusion protein comprising a nickase variant of Cas9 and… read more here.
Keywords: base editing; rps5a promoter; crispr; base ... See more keywords
Genome- and transcriptome-wide off-target analyses of an improved cytosine base editor.
Sign Up to like & getrecommendations! Published in 2021 at "Plant physiology"
DOI: 10.1093/plphys/kiab264
Abstract: Cytosine base editors (CBEs) are the promising tools for precise genome editing in plants. It is important to investigate potential off-target effects of an efficient CBE at the genome and transcriptome levels in a major… read more here.
Keywords: cytosine base; control; target; base ... See more keywords
Comparison of gene disruption induced by cytosine base editing‐mediated iSTOP with CRISPR/Cas9‐mediated frameshift
Sign Up to like & getrecommendations! Published in 2020 at "Cell Proliferation"
DOI: 10.1111/cpr.12820
Abstract: Recently developed CRISPR‐dependent cytosine base editor (CBE), converting four codons (CAA, CAG, CGA and TGG) into stop codons without DNA double‐strand breaks (DSB), serves as an efficient gene disruption strategy besides uncontrollable CRISPR‐mediated frameshift. However,… read more here.
Keywords: crispr; mediated frameshift; gene disruption; cytosine base ... See more keywords