Enzyme Prodrug Therapy with Photo-Cross-Linkable Anti-EGFR Affibodies Conjugated to Upconverting Nanoparticles.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.2c02558
Abstract: In this work, we demonstrate that a photo-cross-linkable conjugate of upconverting nanoparticles and cytosine deaminase can catalyze prodrug conversion specifically at tumor sites in vivo. Non-covalent association of proteins and peptides with cellular surfaces leads… read more here.
Keywords: cytosine deaminase; cross linkable; photo cross; upconverting nanoparticles ... See more keywords
APOBEC1 cytosine deaminase activity on single-stranded DNA is suppressed by replication protein A
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa1201
Abstract: Abstract Many APOBEC cytidine deaminase members are known to induce ‘off-target’ cytidine deaminations in 5′TC motifs in genomic DNA that contribute to cancer evolution. In this report, we characterized APOBEC1, which is a possible cancer… read more here.
Keywords: replication protein; cytosine deaminase; apobec1 cytosine; dna ... See more keywords
Intratumoral administration of engineered circRNAs encoding cytosine deaminase-uracil phosphoribosyltransferase (CDUPRT) and IL-15 elicit superior antitumor efficacy.
Sign Up to like & getrecommendations! Published in 2025 at "Molecular cancer therapeutics"
DOI: 10.1158/1535-7163.mct-25-0356
Abstract: Gene-directed enzyme prodrug therapy (GDEPT) represents a promising antitumor strategy owing to its low systemic toxicity. However, clinical translation has been hindered by challenges in suicide gene delivery. Artificially engineered circular RNA (circRNA) demonstrates exceptional… read more here.
Keywords: antitumor efficacy; encoding cytosine; cytosine deaminase; uracil phosphoribosyltransferase ... See more keywords
Engineering the Active Site Lid Dynamics to Improve the Catalytic Efficiency of Yeast Cytosine Deaminase
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24076592
Abstract: Conformational dynamics is important for enzyme catalysis. However, engineering dynamics to achieve a higher catalytic efficiency is still challenging. In this work, we develop a new strategy to improve the activity of yeast cytosine deaminase… read more here.
Keywords: catalytic efficiency; cytosine deaminase; site lid; active site ... See more keywords