Advances in peptide-mediated cytosolic delivery of proteins.
Sign Up to like & getrecommendations! Published in 2021 at "Advanced drug delivery reviews"
DOI: 10.1016/j.addr.2021.02.003
Abstract: The number of protein-based drugs is exponentially increasing. However, development of protein therapeutics against intracellular targets is hampered by the lack of efficient cytosolic delivery strategies. In recent years, the use of cell-penetrating peptides has… read more here.
Keywords: cytosolic delivery; peptide mediated; delivery proteins; delivery ... See more keywords
Cytosolic Delivery of Small Protein Scaffolds Enables Efficient Inhibition of Ras and Myc.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular pharmaceutics"
DOI: 10.1021/acs.molpharmaceut.1c00798
Abstract: The ability to deliver small protein scaffolds intracellularly could enable the targeting and inhibition of many therapeutic targets that are not currently amenable to inhibition with small-molecule drugs. Here, we report the engineering of small… read more here.
Keywords: small protein; cytosolic delivery; inhibition; protein scaffolds ... See more keywords
Discovery of a Cyclic Cell-Penetrating Peptide with Improved Endosomal Escape and Cytosolic Delivery Efficiency.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular pharmaceutics"
DOI: 10.1021/acs.molpharmaceut.1c00924
Abstract: Cyclic cell-penetrating peptide 12 (CPP12) is highly efficient for the cytosolic delivery of a variety of cargo molecules into mammalian cells in vitro and in vivo. However, its cytosolic entry efficiency is substantially reduced at… read more here.
Keywords: cell penetrating; cytosolic delivery; penetrating peptide; cyclic cell ... See more keywords
Cellular Uptake and Cytosolic Delivery of a Cyclic Cystine Knot Scaffold.
Sign Up to like & getrecommendations! Published in 2020 at "ACS chemical biology"
DOI: 10.1021/acschembio.0c00297
Abstract: Cyclotides are macrocyclic peptides that have exceptionally stable structures and been reported to penetrate cells, making them promising scaffolds for the delivery of peptide inhibitory sequences to target intracellular proteins. However, their cellular uptake and… read more here.
Keywords: cytosolic delivery; delivery cyclic; cyclic cystine; delivery ... See more keywords
Targeted Protein Degradation through Cytosolic Delivery of Monobody Binders Using Bacterial Toxins
Sign Up to like & getrecommendations! Published in 2019 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.9b00113
Abstract: Monobodies are small engineered binding proteins that, upon expression in cells, can inhibit signaling of cytosolic oncoproteins with outstanding selectivity. Efficacy may be further increased by inducing degradation of monobody targets through fusion to the… read more here.
Keywords: cytosolic delivery; monobody; delivery; protein degradation ... See more keywords
Direct Cytosolic Delivery of Proteins Through Co-Engineering of Proteins and Polymeric Delivery Vehicles.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b12759
Abstract: Nanocarrier-mediated protein delivery is a promising strategy for fundamental research and therapeutic applications. However, the efficacy of current platforms for delivery into cells is limited by endosomal entrapment of delivered protein cargo, with concomitantly inefficient… read more here.
Keywords: cytosolic delivery; proteins engineering; delivery proteins; delivery ... See more keywords
Cytosolic delivery: Just passing through.
Sign Up to like & getrecommendations! Published in 2017 at "Nature chemistry"
DOI: 10.1038/nchem.2837
Abstract: Intracellular protein delivery has been a major challenge in the field of cell biology for decades. Engineering such delivery is a key step in the development of protein- and antibody-based therapeutics. Now, two different approaches… read more here.
Keywords: cytosolic delivery; delivery; chemistry; delivery passing ... See more keywords
Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-23997-x
Abstract: Cytosolic transport is an essential requirement but a major obstacle to efficient delivery of therapeutic peptides, proteins and nucleic acids. Current understanding of cytosolic delivery mechanisms remains limited due to a significant number of conflicting… read more here.
Keywords: cytosolic delivery; cell penetrating; delivery; endosomal escape ... See more keywords
Recent progress in nanomedicine-mediated cytosolic delivery
Sign Up to like & getrecommendations! Published in 2023 at "RSC Advances"
DOI: 10.1039/d2ra07111h
Abstract: Cytosolic delivery of bioactive agents has exhibited great potential to cure undruggable targets and diseases. Because biological cell membranes are a natural barrier for living cells, efficient delivery methods are required to transfer bioactive and… read more here.
Keywords: recent progress; cytosolic delivery; nanomedicine mediated; mediated cytosolic ... See more keywords