Detecting potato viruses using direct reverse transcription quantitative PCR (DiRT-qPCR) without RNA purification: an alternative to DAS-ELISA
Sign Up to like & getrecommendations! Published in 2018 at "European Journal of Plant Pathology"
DOI: 10.1007/s10658-018-1468-x
Abstract: Virus screening is obligatory to avoid the spread of plant viruses regionally and globally. Double-antibody sandwich (DAS)-ELISA is the standard for screening potato viruses owing to its high-throughput potential, robustness, and cost–benefit ratio. However, low… read more here.
Keywords: dirt qpcr; potato; virus; das elisa ... See more keywords
Establishment of DAS-ELISA for the detection of antigenic changes in glycinin after heat processing.
Sign Up to like & getrecommendations! Published in 2022 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2022.03.205
Abstract: In this study, a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) method was established to detect the antigenic changes of thermally processed products containing glycinin. The proposed DAS-ELISA method used heat-treated antigen-absorbing antiserum as the… read more here.
Keywords: das elisa; heat; method; antibody ... See more keywords
Development of a DAS-ELISA for detection of H9N2 avian influenza virus.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2018.10.014
Abstract: H9N2 avian influenza virus is threatening animals and public health systems. Effective diagnosis is imperative to control the disease. Thus, we developed a panel of monoclonal antibodies (Mabs) against the H9N2 avian influenza virus (AIV)… read more here.
Keywords: avian influenza; das elisa; h9n2 avian; influenza virus ... See more keywords
Development of a Novel Double Antibody Sandwich ELISA for Quantitative Detection of Porcine Deltacoronavirus Antigen
Sign Up to like & getrecommendations! Published in 2021 at "Viruses"
DOI: 10.3390/v13122403
Abstract: Porcine deltacoronavirus (PDCoV) can cause diarrhea and dehydration in newborn piglets. Here, we developed a double antibody sandwich quantitative enzyme-linked immunosorbent assay (DAS-ELISA) for detection of PDCoV by using a specific monoclonal antibody against the… read more here.
Keywords: pdcov; antigen; detection; antibody ... See more keywords
Development of a DAS–ELISA for Gyrovirus Homsa1 Prevalence Survey in Chickens and Wild Birds in China
Sign Up to like & getrecommendations! Published in 2023 at "Veterinary Sciences"
DOI: 10.3390/vetsci10050312
Abstract: Simple Summary We developed the DAS–ELISA to investigate the infection and prevalence of GyH1 in chickens and wild birds in China. We found that natural GyH1 infections were widespread in chickens and wild birds. Importantly,… read more here.
Keywords: wild birds; das elisa; chickens wild; gyh1 ... See more keywords