Synthetic Epigenetic Reprogramming of Mesenchymal to Epithelial States Using the CRISPR/dCas9 Platform in Triple Negative Breast Cancer.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced science"
DOI: 10.1002/advs.202301802
Abstract: Epithelial-mesenchymal transition (EMT) is a reversible transcriptional program invoked by cancer cells to drive cancer progression. Transcription factor ZEB1 is a master regulator of EMT, driving disease recurrence in poor-outcome triple negative breast cancers (TNBCs). Here,… read more here.
Keywords: breast; triple negative; dcas9; zeb1 ... See more keywords
Generation of a dual edited human induced pluripotent stem cell Myl7-GFP reporter line with inducible CRISPRi/dCas9.
Sign Up to like & getrecommendations! Published in 2022 at "Stem cell research"
DOI: 10.1016/j.scr.2022.102754
Abstract: Temporal regulation of CRISPRi activity is critical for genetic screens. Here, we present an inducible CRISPRi platform enabling selection of iPSC-derived cardiomyocytes and reversible gene knockdown. We targeted a doxycycline-inducible dCas9-KRAB-mCherry cassette into the AAVS1… read more here.
Keywords: myl7; dcas9; ipsc; stem cell ... See more keywords
dCas9-Mediated PCR-Free Detection of Oncogenic Mutation by Nonequilibrium Nanoelectrokinetic Selective Preconcentration.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c05539
Abstract: Cutting-edge nanoelectrokinetic technology in this work provides a breakthrough for the present clinical demands of molecular diagnosis to detect a trace amount of oncogenic mutation of DNA in a short time without an erroneous PCR… read more here.
Keywords: dna; oncogenic mutation; dcas9 mediated; detection ... See more keywords
Capturing ctDNA from Unaltered Stationary and Flowing Plasma with dCas9.
Sign Up to like & getrecommendations! Published in 2022 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.2c03186
Abstract: Many studies have established that blood-based liquid biopsies can be used to detect cancer in its early stages. However, the limiting factor for early cancer detection is the volume of blood required to capture the… read more here.
Keywords: ctdna; plasma; dcas9; capture ... See more keywords
Small-Molecule-Mediated Split-Aptamer Assembly for Inducible CRISPR-dCas9 Transcription Activation.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00101
Abstract: Inducible CRISPR-dCas9 transcription system has become a powerful tool for transcription regulation and sensing. Here, we develop a new concept of small-molecule-mediated split-aptamer assembly for inducible CRISPR-dCas9 transcription activation, allowing quantitative detection and imaging of… read more here.
Keywords: inducible crispr; dcas9 transcription; transcription; dcas9 ... See more keywords
Host Cell Transcriptional Tuning with CRISPR/dCas9 to Mitigate the Effects of Toxin Exposure.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00214
Abstract: Anthrax infection is caused byBacillus anthracis, a bacterium that once established within the host releases lethal toxin (LeTx). Anthrax LeTx is internalized by the capillary morphogenesis protein 2/anthrax toxin receptor 2 (CMG2/ANTXR2) cell surface receptor… read more here.
Keywords: cell; toxin; antxr2; dcas9 ... See more keywords
Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2017 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.7b00163
Abstract: Standard approaches for dCas9-based modification of gene expression are limited in the ability to multiplex targets, establish streamlined cassettes, and utilize commonly studied Pol II promoters. In this work, we repurpose the dCas9-VPR activator to… read more here.
Keywords: dual mode; dcas9; multiplex; activator ... See more keywords
Reprogrammable CRISPR/dCas9-based recruitment of DNMT1 for site-specific DNA demethylation and gene regulation
Sign Up to like & getrecommendations! Published in 2019 at "Cell Discovery"
DOI: 10.1038/s41421-019-0090-1
Abstract: Dear Editor, DNA methylation and demethylation play a critical role in regulating gene expression as well as developmental and pathological processes. For example, hypermethylation in the promoter region typically represses a gene’s transcription, and aberrant… read more here.
Keywords: methylation; dcas9; crispr; dna ... See more keywords
Highly efficient activation of endogenous gene in grape using CRISPR/dCas9-based transcriptional activators
Sign Up to like & getrecommendations! Published in 2022 at "Horticulture Research"
DOI: 10.1093/hr/uhab037
Abstract: Abstract Overexpression and knockout (or knockdown) of gene of interest are two commonly used strategies for gene functional study. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system-mediated gene knockout has been applied in most plant… read more here.
Keywords: grape; activation; based transcriptional; gene ... See more keywords
Antagonistic and synergistic epigenetic modulation using orthologous CRISPR/dCas9-based modular system
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz709
Abstract: Abstract Establishing causal relationship between epigenetic marks and gene transcription requires molecular tools, which can precisely modify specific genomic regions. Here, we present a modular and extensible CRISPR/dCas9-based toolbox for epigenetic editing and direct gene… read more here.
Keywords: dcas9; system; dcas9 based; gene ... See more keywords
Transcriptional activation of endogenous Oct4 via the CRISPR/dCas9 activator ameliorates Hutchinson-Gilford progeria syndrome in mice.
Sign Up to like & getrecommendations! Published in 2023 at "Aging cell"
DOI: 10.1111/acel.13825
Abstract: Partial cellular reprogramming via transient expression of Oct4, Sox2, Klf4, and c-Myc induces rejuvenation and reduces aged-cell phenotypes. In this study, we found that transcriptional activation of the endogenous Oct4 gene by using the CRISPR/dCas9… read more here.
Keywords: transcriptional activation; dcas9 activator; dcas9; crispr dcas9 ... See more keywords