Human cleaving embryos enable efficient mitochondrial base-editing with DdCBE
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DOI: 10.1038/s41421-021-00372-0
Abstract: Dear Editor, Mitochondrial diseases could be caused by heritable mutations in both mtDNA and nuclear DNA. Because mitochondria membrane insulated mtDNAs’ access from large ribonucleoprotein complex of Cas9 and sgRNA, mtDNAs are intractable for genetic… read more here.
Keywords: human embryos; embryos; conversion; ddcbe ... See more keywords
Mitochondrial base editor DdCBE causes substantial DNA off-target editing in nuclear genome of embryos
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DOI: 10.1038/s41421-022-00391-5
Abstract: Dear Editor, Mitochondrial DNA (mtDNA) is encapsulated by the organelle membrane forming the barrier for the access of CRISPR-based gene-editing tools to the mtDNA. Furthermore, mitochondrial genome lacks similar repair systems for the protection of… read more here.
Keywords: dna; nuclear genome; ddcbe; target ... See more keywords