Next-generation molecular diagnostics (TaqMan qPCR and ddPCR) for monitoring insecticide resistance in Bemisia tabaci.
Sign Up to like & getrecommendations! Published in 2022 at "Pest management science"
DOI: 10.1002/ps.7122
Abstract: BACKGROUND Insecticide resistance has developed in several populations of the whitefly Bemisia tabaci worldwide and threatens to compromise the efficacy of chemical control. The molecular mechanisms underpinning resistance have been characterized and markers associated with… read more here.
Keywords: ddpcr; bemisia tabaci; insecticide resistance; resistance ... See more keywords
Development and application of a sensitive droplet digital PCR (ddPCR) for the detection of infectious spleen and kidney necrosis virus
Sign Up to like & getrecommendations! Published in 2020 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2020.735697
Abstract: Abstract Infectious spleen and kidney necrosis, caused by the infectious spleen and kidney necrosis virus (ISKNV), has damaged the economy of the world fish industry. Droplet digital PCR (ddPCR) is a novel, sensitive, accurate and… read more here.
Keywords: ddpcr; detection; spleen kidney; kidney necrosis ... See more keywords
Quantitative detection of viable but nonculturable state Escherichia coli O157:H7 by ddPCR combined with propidium monoazide
Sign Up to like & getrecommendations! Published in 2020 at "Food Control"
DOI: 10.1016/j.foodcont.2020.107140
Abstract: Abstract Escherichia coli O157:H7, the causative agent of haemorrhagic colitis and haemolytic uremic syndrome in humans, has been implicated in large food-borne outbreaks all over the world. When confronted with harsh environmental stresses, it can… read more here.
Keywords: ddpcr; escherichia coli; coli o157; coli ... See more keywords
Droplet digital PCR as a novel detection method for quantifying microRNAs in acute myocardial infarction.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of cardiology"
DOI: 10.1016/j.ijcard.2017.10.111
Abstract: BACKGROUND micro-RNAs have shown promise as potential biomarkers for acute myocardial infarction and ischemia-reperfusion injury (I/R). Most recently droplet digital polymerase chain reaction (ddPCR) has been introduced as a more reliable and reproducible method for… read more here.
Keywords: ddpcr; pcr; myocardial infarction; qrt pcr ... See more keywords
Determination of SARS-CoV-2 Contamination in a Neonatal Intensive Care Unit (NICU) Environment Using Droplet Digital PCR (ddPCR)
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Infectious Diseases"
DOI: 10.1016/j.ijid.2021.12.125
Abstract: Purpose Neonatal infections with SARS-CoV-2 are thought to be less contagious than in older children and adults. The transmission of SARS-CoV-2 from neonates and their environment has not been well studied. Droplet Digital PCR (ddPCR)… read more here.
Keywords: incubator; droplet; sars cov; environment ... See more keywords
Optimisation of droplet digital PCR for determining copy number variation of α-gliadin genes in mutant and gene-edited polyploid bread wheat
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Cereal Science"
DOI: 10.1016/j.jcs.2019.102903
Abstract: Targeted and random mutagenesis of gene families require accurate quantification. Droplet digital PCR (ddPCR) enables high-throughput screening of copy number variation (CNV). We tested the accuracy of ddPCR for CNV analysis in the large α-gliadin… read more here.
Keywords: ddpcr; gliadin genes; droplet digital; wheat ... See more keywords
Droplet digital PCR applications in the tuberculosis world.
Sign Up to like & getrecommendations! Published in 2019 at "Tuberculosis"
DOI: 10.1016/j.tube.2019.07.001
Abstract: Droplet digital polymerase chain reaction (ddPCR) is a third generation of polymerase chain reaction (PCR) that enables the exact quantification of nucleic acid targets within a sample. The capability of ddPCR to accurately detect and… read more here.
Keywords: applications tuberculosis; droplet digital; pcr; tuberculosis ... See more keywords
Insight & Development of Advanced rAAV Analysis Tools Exploiting Single Particle Quantification by Multidimensional ddPCR.
Sign Up to like & getrecommendations! Published in 2021 at "Human gene therapy"
DOI: 10.1089/hum.2021.182
Abstract: Recombinant Adeno-Associated Virus (rAAV) has become the most widely used vector in the gene therapy field with hundreds of clinical trials ongoing and already several products on the market. AAV's physico-chemical stability, and the various… read more here.
Keywords: quantification; insight development; development advanced; ddpcr ... See more keywords
Development and application of a sensitive droplet digital PCR-based method to detect tilapia parvovirus.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of fish diseases"
DOI: 10.1111/jfd.13738
Abstract: Tilapia parvovirus (TiPV) causes severe mortality rates in cultured tilapia, resulting in substantial losses to the fish industry. Droplet digital PCR (ddPCR) is a sensitive, accurate, and absolute quantitation method, plus it does not require… read more here.
Keywords: application; ddpcr; tilapia parvovirus; pcr ... See more keywords
Respiratory Adenovirus Quantification with a Droplet Digital Polymerase Chain Reaction (ddPCR) Assay
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00269-23
Abstract: Measuring viral load using droplet digital PCR (ddPCR) is an absolute quantification approach that can facilitate comparability between different laboratories. This approach could prove valuable in studies that focus on the clinical utility of quantification.… read more here.
Keywords: quantification; ddpcr; hadv; droplet digital ... See more keywords
Development of Droplet Digital PCR-Based Assays to Quantify HIV Proviral and Integrated DNA in Brain Tissues from Viremic Individuals with Encephalitis and Virally Suppressed Aviremic Individuals
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00853-21
Abstract: We developed ddPCR assays to quantitatively measure HIV DNA and used this ddPCR assays to detect and quantitatively measure HIV DNA in the archived brain tissues from HIV patients. The tissue viral loads assessed by… read more here.
Keywords: dna; brain tissues; hiv dna; ddpcr ... See more keywords