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   Articles with "ddpcr" as a keyword



Quantifying the Detection Sensitivity and Precision of qPCR and ddPCR Mechanisms for eDNA Samples

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recommendations! Published in 2024 at "Ecology and Evolution"

DOI: 10.1002/ece3.70678

Abstract: ABSTRACT Environmental DNA (eDNA) detection employing quantitative PCR (qPCR) and droplet digital PCR (ddPCR) offers a non‐invasive and efficient approach for monitoring aquatic organisms. Accurate and sensitive quantification of eDNA is crucial for tracking rare… read more here.

Keywords: detection; ddpcr; precision qpcr; quantification ... See more keywords

Droplet Digital PCR Provides Highly Sensitive and Accurate Opsin Gene SNP Detection From Wild Primate Fecal Samples

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recommendations! Published in 2025 at "Ecology and Evolution"

DOI: 10.1002/ece3.70996

Abstract: ABSTRACT Evaluating field‐sourced samples with poor‐quality and low‐quantity DNA, like animal feces, presents significant challenges in the field of molecular biology. Nonetheless, recent innovations in PCR technology are promoted as effective tools to overcome many… read more here.

Keywords: droplet digital; ddpcr; pcr; digital pcr ... See more keywords

Next-generation molecular diagnostics (TaqMan qPCR and ddPCR) for monitoring insecticide resistance in Bemisia tabaci.

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recommendations! Published in 2022 at "Pest management science"

DOI: 10.1002/ps.7122

Abstract: BACKGROUND Insecticide resistance has developed in several populations of the whitefly Bemisia tabaci worldwide and threatens to compromise the efficacy of chemical control. The molecular mechanisms underpinning resistance have been characterized and markers associated with… read more here.

Keywords: ddpcr; bemisia tabaci; insecticide resistance; resistance ... See more keywords

Development and application of a sensitive droplet digital PCR (ddPCR) for the detection of infectious spleen and kidney necrosis virus

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recommendations! Published in 2020 at "Aquaculture"

DOI: 10.1016/j.aquaculture.2020.735697

Abstract: Abstract Infectious spleen and kidney necrosis, caused by the infectious spleen and kidney necrosis virus (ISKNV), has damaged the economy of the world fish industry. Droplet digital PCR (ddPCR) is a novel, sensitive, accurate and… read more here.

Keywords: ddpcr; detection; spleen kidney; kidney necrosis ... See more keywords
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Quantitative detection of viable but nonculturable state Escherichia coli O157:H7 by ddPCR combined with propidium monoazide

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recommendations! Published in 2020 at "Food Control"

DOI: 10.1016/j.foodcont.2020.107140

Abstract: Abstract Escherichia coli O157:H7, the causative agent of haemorrhagic colitis and haemolytic uremic syndrome in humans, has been implicated in large food-borne outbreaks all over the world. When confronted with harsh environmental stresses, it can… read more here.

Keywords: ddpcr; escherichia coli; coli o157; coli ... See more keywords

Droplet digital PCR as a novel detection method for quantifying microRNAs in acute myocardial infarction.

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recommendations! Published in 2018 at "International journal of cardiology"

DOI: 10.1016/j.ijcard.2017.10.111

Abstract: BACKGROUND micro-RNAs have shown promise as potential biomarkers for acute myocardial infarction and ischemia-reperfusion injury (I/R). Most recently droplet digital polymerase chain reaction (ddPCR) has been introduced as a more reliable and reproducible method for… read more here.

Keywords: ddpcr; pcr; myocardial infarction; qrt pcr ... See more keywords

Determination of SARS-CoV-2 Contamination in a Neonatal Intensive Care Unit (NICU) Environment Using Droplet Digital PCR (ddPCR)

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recommendations! Published in 2022 at "International Journal of Infectious Diseases"

DOI: 10.1016/j.ijid.2021.12.125

Abstract: Purpose Neonatal infections with SARS-CoV-2 are thought to be less contagious than in older children and adults. The transmission of SARS-CoV-2 from neonates and their environment has not been well studied. Droplet Digital PCR (ddPCR)… read more here.

Keywords: incubator; droplet; sars cov; environment ... See more keywords

Optimisation of droplet digital PCR for determining copy number variation of α-gliadin genes in mutant and gene-edited polyploid bread wheat

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recommendations! Published in 2020 at "Journal of Cereal Science"

DOI: 10.1016/j.jcs.2019.102903

Abstract: Targeted and random mutagenesis of gene families require accurate quantification. Droplet digital PCR (ddPCR) enables high-throughput screening of copy number variation (CNV). We tested the accuracy of ddPCR for CNV analysis in the large α-gliadin… read more here.

Keywords: ddpcr; gliadin genes; droplet digital; wheat ... See more keywords

Droplet digital PCR applications in the tuberculosis world.

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recommendations! Published in 2019 at "Tuberculosis"

DOI: 10.1016/j.tube.2019.07.001

Abstract: Droplet digital polymerase chain reaction (ddPCR) is a third generation of polymerase chain reaction (PCR) that enables the exact quantification of nucleic acid targets within a sample. The capability of ddPCR to accurately detect and… read more here.

Keywords: applications tuberculosis; droplet digital; pcr; tuberculosis ... See more keywords

Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses

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recommendations! Published in 2025 at "Scientific Reports"

DOI: 10.1038/s41598-024-81682-7

Abstract: BPV1, BPV2, BPV13, and BPV14 are all genotypes of bovine delta papillomaviruses (δPV), of which the first three cause infections in horses and are associated with equine sarcoids. However, BPV14 infection has never been reported… read more here.

Keywords: bpv14; ddpcr; qpcr; semen samples ... See more keywords

Sensitive and rapid identification of pathogens by droplet digital PCR in a cohort of septic patients: a prospective diagnostic study

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recommendations! Published in 2024 at "Infectious Diseases"

DOI: 10.1080/23744235.2024.2354312

Abstract: Abstract Background There is a critical need for a rapid and sensitive pathogen detection method for septic patients. This study aimed to investigate the diagnostic efficacy of Digital droplet polymerase chain reaction (ddPCR) in identifying… read more here.

Keywords: diagnostic study; septic patients; ddpcr; culture ... See more keywords