Development and validation of a droplet digital PCR assay for the detection and quantification of Bartonella species within human clinical samples.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2020.106022
Abstract: This report describes the development, optimization, and validation of a ddPCR assay for the detection of Bartonella spp. DNA within several sample matrices, including clinical blood samples from patients with or without documented Bartonella spp.… read more here.
Keywords: ddpcr assay; bartonella; bartonella spp; development ... See more keywords
Development and comparative evaluation of droplet digital PCR and quantitative PCR for the detection and quantification of Chlamydia psittaci.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2021.106318
Abstract: Chlamydia psittaci is a zoonotic pathogen mainly transmitted by psittacine birds and poultry. The low shedding rate of the pathogen in the apparently healthy birds and human clinical cases may result in false-negative results. In… read more here.
Keywords: detection; droplet digital; ddpcr assay; pcr ... See more keywords
Development and Analytical Validation of a One-Step Five-Plex RT-ddPCR Assay for the Quantification of SARS-CoV-2 Transcripts in Clinical Samples
Sign Up to like & getrecommendations! Published in 2022 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c00868
Abstract: Highly sensitive methodologies for SARS-CoV-2 detection are essential for the control of COVID-19 pandemic. We developed and analytically validated a highly sensitive and specific five-plex one-step RT-ddPCR assay for SARS-CoV-2. We first designed in-silico novel… read more here.
Keywords: quantification; one step; sars cov; five plex ... See more keywords
A Novel Reverse Transcription Droplet Digital PCR Assay for the Sensitive Detection of Combing Severe Acute Respiratory Syndrome Coronavirus 2 with Swine Acute Diarrhea Syndrome Coronavirus.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of AOAC International"
DOI: 10.1093/jaoacint/qsac039
Abstract: BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread over the world since its emergence. Although the dominant route of SARS-CoV-2 infection is respiratory, a number of studies revealed infectious risk from contaminated… read more here.
Keywords: sads cov; syndrome coronavirus; sars cov; detection ... See more keywords
Respiratory Adenovirus Quantification with a Droplet Digital Polymerase Chain Reaction (ddPCR) Assay
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00269-23
Abstract: Measuring viral load using droplet digital PCR (ddPCR) is an absolute quantification approach that can facilitate comparability between different laboratories. This approach could prove valuable in studies that focus on the clinical utility of quantification.… read more here.
Keywords: quantification; ddpcr; hadv; droplet digital ... See more keywords