Site‐specific rapid deamidation and isomerization in human lens αA‐crystallin in vitro
Sign Up to like & getrecommendations! Published in 2020 at "Protein Science"
DOI: 10.1002/pro.3821
Abstract: Recent studies have suggested that the isomerization/racemization of aspartate residues in proteins increases in aged tissues. One such residue is Asp151 in lens‐specific αA‐crystallin. Although many isomerization/racemization sites have been reported in various proteins, the… read more here.
Keywords: deamidation isomerization; crystallin; deamidation; isomerization racemization ... See more keywords
Backbone assignments, and effect of Asn deamidation, of the N-terminal region of the partitioning protein IncC1 from the plasmid RK2
Sign Up to like & getrecommendations! Published in 2021 at "Biomolecular Nmr Assignments"
DOI: 10.1007/s12104-021-10021-y
Abstract: IncC from the low-copy number plasmid RK2, is a member of the ParA family of proteins required for partitioning DNA in many bacteria and plasmids. It is an ATPase that binds DNA and its ParB… read more here.
Keywords: protein; amino acids; backbone; deamidation ... See more keywords
Post-translational modifications in collagen type I of bone in a mouse model of aging.
Sign Up to like & getrecommendations! Published in 2020 at "Bone"
DOI: 10.1016/j.bone.2020.115763
Abstract: The fracture resistance of cortical bone and matrix hydration are known to decline with advanced aging. However, the underlying mechanisms remain poorly understood, and so we investigated levels of matrix proteins and post-translational modifications (PTM)… read more here.
Keywords: collagen; age; bone; deamidation ... See more keywords
Effect of nonenzymatic deamidation on the structure stability of Camelus dromedarius α-lactalbumin.
Sign Up to like & getrecommendations! Published in 2019 at "Food chemistry"
DOI: 10.1016/j.foodchem.2019.04.033
Abstract: Camelid α-lactalbumin is the only known protein that can undergo nonenzymatic deamidation on two Asn residues. This leads to the generation of a mixture of unusual isoAsp and d-Asp residues that may impact health. The… read more here.
Keywords: stability; nonenzymatic deamidation; effect; lactalbumin ... See more keywords
The use of microbial transglutaminase in a bread system: A study of gluten protein structure, deamidation state and protein digestion.
Sign Up to like & getrecommendations! Published in 2020 at "Food chemistry"
DOI: 10.1016/j.foodchem.2020.127903
Abstract: Microbial transglutaminase (mTG) catalyses the formation of protein crosslinks, deamidating glutamine in a side-reaction. Gluten deamidation by human tissue transglutaminase is critical to activate celiac disease pathogenesis making the addition of mTG to wheat-based products… read more here.
Keywords: protein; deamidation state; transglutaminase; mtg ... See more keywords
Effect of pH and heat treatment on physicochemical and functional properties of spray-dried whey protein concentrate powder
Sign Up to like & getrecommendations! Published in 2021 at "International Dairy Journal"
DOI: 10.1016/j.idairyj.2021.105063
Abstract: Abstract Effects of pH and heating on deamidation of whey protein concentrate (WPC) solution and functional properties of resultant spray-dried WPC powder were investigated. Temperature and heating time affected deamidation rates with the highest reactivities… read more here.
Keywords: properties spray; functional properties; spray dried; deamidation ... See more keywords
Deamidation in Moxetumomab Pasudotox Leading to Conformational Change and Immunotoxin Activity Loss.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical sciences"
DOI: 10.1016/j.xphs.2020.06.002
Abstract: Asparagine deamidation is a common posttranslational modification in which asparagine (Asn) is converted to aspartic acid or isoaspartic acid. By introducing a negative charge, deamidation could potentially impact the binding interface and biological activities of… read more here.
Keywords: deamidation; activity; moxetumomab pasudotox; immunotoxin ... See more keywords
Introducing protein deamidation: landmark discoveries, societal outreach, and tentative priming workflow to address deamidation.
Sign Up to like & getrecommendations! Published in 2021 at "Methods"
DOI: 10.1016/j.ymeth.2021.11.012
Abstract: Our current knowledge on protein deamidation results from a journey that started almost 100 years ago, when a handful of researchers first described the non-enzymatic "desamidation" of glutamine, and the effect of different anions on… read more here.
Keywords: landmark discoveries; protein deamidation; biology; deamidation ... See more keywords
Standard-Free Absolute Quantitation of Antibody Deamidation Degradation and Host Cell Proteins by Coulometric Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02709
Abstract: Proteomic absolute quantitation strategies mainly rely on the use of synthetic stable isotope-labeled peptides or proteins as internal standards, which are highly costly and time-consuming to synthesize. To circumvent this limitation, we recently developed a… read more here.
Keywords: deamidation; mass spectrometry; absolute quantitation; cms ... See more keywords
Pertuzumab Charge Variant Analysis and Complementarity-Determining Region Stability Assessment to Deamidation
Sign Up to like & getrecommendations! Published in 2023 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c03275
Abstract: Pertuzumab is a monoclonal antibody used for the treatment of HER2-positive breast cancer in combination with trastuzumab. Charge variants of trastuzumab have been extensively described in the literature; however, little is known about the charge… read more here.
Keywords: heavy chain; stress; deamidation; charge ... See more keywords
Robust High-Throughput Proteomics Identification and Deamidation Quantitation of Extinct Species up to Pleistocene with Ultrahigh-Resolution MALDI-FTICR Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03301
Abstract: Peptide mass fingerprinting (PMF) using MALDI-TOF mass spectrometry allows the identification of bone species based on their type I collagen sequence. In the archaeological or paleontological field, PMF is known as zooarchaeology mass spectrometry (ZooMS)… read more here.
Keywords: deamidation; mass spectrometry; bone; identification ... See more keywords