Optimizing Amplification of the GC-Rich TERT Promoter Region Using 7-Deaza-dGTP for Droplet Digital PCR Quantification of TERT Promoter Mutations.
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DOI: 10.1373/clinchem.2017.284257
Abstract: To the Editor: Droplet digital PCR (ddPCR) assays partition PCR reactions into multiple compartments. PCR amplification followed by analysis of end point fluorescence of each partition allows the absolute quantification of the target sequence. ddPCR… read more here.
Keywords: promoter mutations; tert promoter; deaza dgtp; pcr ... See more keywords