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   Articles with "decalcification" as a keyword



Impact of delayed fixation and decalcification on PD-L1 expression: a comparison of two clones

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recommendations! Published in 2019 at "Virchows Archiv"

DOI: 10.1007/s00428-019-02613-w

Abstract: The bone is a frequent localization for lung non-small cell cancer metastasis; decalcification is required to permit tissue section. Pre-analytical conditions can influence the detection of immunohistochemical markers. The aim of our work is to… read more here.

Keywords: fixation; delayed fixation; decalcification; expression ... See more keywords

Decalcification of a Heavily Calcified Common Femoral Artery and its Bifurcation with a Cavitron Ultrasonic Surgical Aspirator

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recommendations! Published in 2017 at "EJVES Short Reports"

DOI: 10.1016/j.ejvssr.2016.09.003

Abstract: Introduction Surgical endarterectomy is the preferred method for treating occlusive disease of the common femoral artery (CFA). However, endarterectomy is not always straightforward in cases with heavily calcified plaque. To overcome this limitation, a new… read more here.

Keywords: heavily calcified; cavitron ultrasonic; decalcification; femoral artery ... See more keywords

Effect of EDTA Decalcification on ER and PR Immunohistochemistry and HER2/neu Fluorescence In Situ Hybridization in Breast Carcinoma.

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recommendations! Published in 2021 at "Human pathology"

DOI: 10.1016/j.humpath.2021.08.007

Abstract: Bone is the most common site of metastasis in breast carcinoma (BC). Treatments for metastatic BC depend on various factors including the tumor's estrogen receptor (ER), progesterone receptor (PR), and HER2 status. Bone biopsies require… read more here.

Keywords: ihc her2; breast carcinoma; decalcification; her2 fish ... See more keywords

Hypertonic saline- and detergent-accelerated EDTA-based decalcification better preserves mRNA of bones

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recommendations! Published in 2024 at "Scientific Reports"

DOI: 10.1038/s41598-024-61459-8

Abstract: Ethylenediaminetetraacetic acid (EDTA), a classically used chelating agent of decalcification, maintains good morphological details, but its slow decalcification limits its wider applications. Many procedures have been reported to accelerate EDTA-based decalcification, involving temperature, concentration, sonication,… read more here.

Keywords: decalcification; hypertonic saline; edta based; based decalcification ... See more keywords

A comparison of three decalcification agents for assessments of cranial fracture histomorphology

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recommendations! Published in 2022 at "Journal of Forensic Sciences"

DOI: 10.1111/1556-4029.14990

Abstract: The extraction of mineral calcium from bone by decalcification is a critical step in the preparation of histological samples for light microscopy. This study assessed the time required for complete decalcification and the resultant histomorphological… read more here.

Keywords: histomorphology; three decalcification; acid; decalcification ... See more keywords

A Quality Analysis of Bony Specimens for Optimal Ethylenediaminetetraacetic Acid (EDTA) Decalcification

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recommendations! Published in 2022 at "International Journal of Surgical Pathology"

DOI: 10.1177/10668969221088877

Abstract: Introduction: Weak acids, such as etheylenediaminetetraacetic acid (EDTA), chelate calcium ions from the surface of tissues, decalcifying slowly but preserving molecular structures for ancillary testing. There is a need for optimization of EDTA protocols for… read more here.

Keywords: edta; decalcification; histology; bony specimens ... See more keywords

Decalcify cardiac CT: unveiling clearer images with deep convolutional neural networks

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recommendations! Published in 2025 at "Frontiers in Medicine"

DOI: 10.3389/fmed.2025.1475362

Abstract: Decalcification is crucial in enhancing the diagnostic accuracy and interpretability of cardiac CT images, particularly in cardiovascular imaging. Calcification in the coronary arteries and cardiac structures can significantly impact the quality of the images and… read more here.

Keywords: decalcification; unveiling clearer; cardiac unveiling; cardiac images ... See more keywords

Arduino Automated Microwave Oven for Tissue Decalcification

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recommendations! Published in 2023 at "Bioengineering"

DOI: 10.3390/bioengineering10010079

Abstract: Decalcification of hard tissues such as bone and teeth is a complex process that requires using chemicals such as acids and chelating agents. Acids act faster than chelating agents, but they have a greater risk… read more here.

Keywords: arduino automated; microwave oven; decalcification; automated microwave ... See more keywords

Identification of Optimal Decalcification Method and Tissue Preparation Protocol for RNAscope In Situ Hybridization in Rodent Incisor Tooth

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recommendations! Published in 2025 at "Dentistry Journal"

DOI: 10.3390/dj13110538

Abstract: Background: RT-qPCR is the gold standard for quantitative gene expression analysis, but it requires homogenized tissue and thus loses spatial information. RNA in situ hybridization (ISH) preserves tissue localization but is technically challenging, especially in… read more here.

Keywords: decalcification; optimal decalcification; tooth; situ hybridization ... See more keywords

ULTRASONIC DECALCIFICATION OF AORTIC VALVE IN MODERN ERA: A CASE REPORT.

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recommendations! Published in 2022 at "Wiadomosci lekarskie"

DOI: 10.36740/wlek202203128

Abstract: Aortic stenosis (AS) - a constantly progressing disease characterized by thickening and calcification of leaflets of the valve, which leads to obstruction of the blood outflow from the left ventricular (LV), inadequate cardiac output, heart… read more here.

Keywords: aortic valve; valve; modern era; report ... See more keywords

A novel approach to decalcification in histopathology laboratory: An adaptation from the Hammersmith protocol

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recommendations! Published in 2019 at "Indian Journal of Pathology and Microbiology"

DOI: 10.4103/ijpm.ijpm_853_18

Abstract: Aim: Utility of modified Hammersmith protocol in the deacalcification and/or softening of tissues and samples in a histopathology laboratory were studied. The object of the study was to prepare a novel method for softening/decalcifying tissue… read more here.

Keywords: pathology; histopathology laboratory; hammersmith protocol; histopathology ... See more keywords