A new strategy for the decellularization of whole organs by hydrostatic pressure
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3248
Abstract: Tissue engineering has been able to develop novel decellularization‐recellularization techniques, which facilitates the research for the generation of functional organs. This is based in the initial obtention of the organ's extracellular matrix (ECM). Therefore, any… read more here.
Keywords: ecm; new strategy; hydrostatic pressure; decellularization ... See more keywords
Formulation of Dermal Tissue Matrix Bioink By A Facile Decellularization Method and Process Optimization for 3D Bioprinting towards Translation Research.
Sign Up to like & getrecommendations! Published in 2022 at "Macromolecular bioscience"
DOI: 10.1002/mabi.202200109
Abstract: Decellularized extracellular matrices are being extensively used for tissue engineering purposes and detergents are predominantly used for this. We have developed a facile detergent-free decellularization method for dermal matrix and compared it with the most… read more here.
Keywords: process optimization; matrix; decellularization method; decellularization ... See more keywords
Pilot study of a novel vacuum‐assisted method for decellularization of tracheae for clinical tissue engineering applications
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Tissue Engineering and Regenerative Medicine"
DOI: 10.1002/term.1979
Abstract: Tissue engineered tracheae have been successfully implanted to treat a small number of patients on compassionate grounds. The treatment has not become mainstream due to the time taken to produce the scaffold and the resultant… read more here.
Keywords: vacuum assisted; time; tissue; vacuum ... See more keywords
Decellularization Concept in Regenerative Medicine.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in experimental medicine and biology"
DOI: 10.1007/5584_2019_338
Abstract: Decellularized organs and tissues are effectively utilized in a diversity of regenerative medicine purposes, and the decellularization approaches employed differ as broadly as the tissues/organs of concern. Biological scaffold substances formed by extracellular matrix (ECM)… read more here.
Keywords: medicine; decellularization concept; biology; decellularization ... See more keywords
Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/7651_2017_31
Abstract: Decellularization of whole porcine hearts followed by recellularization with fully differentiated cells made from patient-specific human induced pluripotent stem cells (iPSCs) may provide the ultimate solution for patients with heart failure. Decellularization is the process… read more here.
Keywords: heart; whole porcine; extracellular matrix; tissue ... See more keywords
Modification of Rat Lung Decellularization Protocol Based on Dynamic Conductometry of Working Solution
Sign Up to like & getrecommendations! Published in 2017 at "Bulletin of Experimental Biology and Medicine"
DOI: 10.1007/s10517-017-3692-3
Abstract: We modified the protocol of obtaining of biological scaffolds of rat lungs based on dynamic recording of specific resistivity of working detergent solution (conductometry) during perfusion decellularization. Termination of sodium deoxycholate exposure after attaining ionic… read more here.
Keywords: based dynamic; conductometry; modification rat; protocol ... See more keywords
Comparative study of decellularization techniques to obtain natural extracellular matrix scaffolds of human peripheral-nerve allografts.
Sign Up to like & getrecommendations! Published in 2021 at "Cell and tissue banking"
DOI: 10.1007/s10561-021-09977-x
Abstract: BACKGROUND We hypothesize that adding sonication cycles to the process of decellularization of cadaveric human peripheral nerves will increase the removal of cell debris and myelin sheath, increasing their utility as allografts. METHODS Our aim… read more here.
Keywords: human peripheral; nerve allografts; decellularization; peripheral nerve ... See more keywords
Whole-ovary decellularization generates an effective 3D bioscaffold for ovarian bioengineering
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Assisted Reproduction and Genetics"
DOI: 10.1007/s10815-020-01784-9
Abstract: Purpose To develop a new protocol for whole-ovary decellularization for the production of a 3D bioscaffold suitable for in vitro/ex vivo studies and for the reconstruction of a bioengineered ovary. Methods Porcine ovaries were subjected… read more here.
Keywords: cell; whole ovary; generates effective; decellularization ... See more keywords
Optimizing Decellularization Strategies for the Efficient Production of Whole Rat Kidney Scaffolds.
Sign Up to like & getrecommendations! Published in 2021 at "Tissue engineering and regenerative medicine"
DOI: 10.1007/s13770-021-00339-y
Abstract: BACKGROUND Renal dysfunction remains a global issue, with chronic kidney disease being the 18th most leading cause of death, worldwide. The increased demands in kidney transplants, led the scientific society to seek alternative strategies, utilizing… read more here.
Keywords: production; decellularization strategies; kidney scaffolds; decellularization ... See more keywords
Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2019.11.023
Abstract: The use of hydrogels derived from the extracellular matrix (ECM) in tissue engineering applications aims to overcome the conundrum of mimicking the complexity of ECM composition in vitro. In this chapter, we describe a method… read more here.
Keywords: porcine heart; extracellular matrix; tissue; heart tissue ... See more keywords
Inverted orientation improves decellularization of whole porcine hearts.
Sign Up to like & getrecommendations! Published in 2017 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2016.11.047
Abstract: In structurally heterogeneous organs, such as heart, it is challenging to retain extracellular matrix integrity in the thinnest regions (eg, valves) during perfusion decellularization and completely remove cellular debris from thicker areas. The high inflow… read more here.
Keywords: heart; orientation; extracellular matrix; integrity ... See more keywords