Multiregion ultra‐deep sequencing reveals early intermixing and variable levels of intratumoral heterogeneity in colorectal cancer
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12012
Abstract: Intratumor heterogeneity (ITH) contributes to cancer progression and chemoresistance. We sought to comprehensively describe ITH of somatic mutations, copy number, and transcriptomic alterations involving clinically and biologically relevant gene pathways in colorectal cancer (CRC). We… read more here.
Keywords: ultra deep; deep sequencing; colorectal cancer; heterogeneity ... See more keywords
Screening for differentially expressed microRNAs in BALF and blood samples of infected COVID‐19 ARDS patients by small RNA deep sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24672
Abstract: The pandemic COVID‐19 has caused a high mortality rate and poses a significant threat to the population of the entire world. Due to the novelty of this disease, the pathogenic mechanism of the disease and… read more here.
Keywords: screening differentially; rna deep; differentially expressed; ards patients ... See more keywords
The Isolation and Deep Sequencing of Mitochondrial DNA.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1270-5_27
Abstract: In recent years, next-generation sequencing (NGS) has become a powerful tool for studying both inherited and somatic heteroplasmic mitochondrial DNA (mtDNA) variation. NGS has proved particularly powerful when combined with single-cell isolation techniques, allowing the… read more here.
Keywords: dna; isolation deep; deep sequencing; sequencing mitochondrial ... See more keywords
Diagnosis of Viral Diseases Using Deep Sequencing and Metagenomics Analyses.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1835-6_22
Abstract: Viruses are ubiquitous in nature and exist in a variety of habitats. The advancement in sequencing technologies has revolutionized the understanding of viral biodiversity associated with plant diseases. Deep sequencing combined with metagenomics is a… read more here.
Keywords: deep sequencing; using deep; sequencing; diseases using ... See more keywords
Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7339-2_3
Abstract: Identification of sequences preferred by individual RNA-binding proteins (RBPs) has been accelerated by recent advances in the quantitative analysis of protein-RNA interactions on a massive scale, and such experiments have even revealed hidden sequence specificity… read more here.
Keywords: single stranded; vitro loading; stranded rnas; deep sequencing ... See more keywords
Workup of Human Blood Samples for Deep Sequencing of HIV-1 Genomes.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7683-6_5
Abstract: We describe a detailed protocol for the manual workup of blood (plasma/serum) samples from individuals infected with the human immunodeficiency virus type 1 (HIV-1) for deep sequence analysis of the viral genome. The study optimizing… read more here.
Keywords: workup human; hiv; blood samples; human blood ... See more keywords
Identification and characterization of the Chinese giant salamander (Andrias davidianus) miRNAs by deep sequencing and predication of their targets
Sign Up to like & getrecommendations! Published in 2017 at "3 Biotech"
DOI: 10.1007/s13205-017-0817-3
Abstract: MicroRNAs (miRNAs) are non-coding small RNA of approximately 22 nt in length. They are considered as key regulators for gene expression at the post-transcriptional level and play an important role in the regulation of many… read more here.
Keywords: davidianus; andrias davidianus; davidianus mirnas; deep sequencing ... See more keywords
Identification and characterization of microRNAs in a cestode Hydatigera taeniaeformis using deep sequencing approach.
Sign Up to like & getrecommendations! Published in 2018 at "Experimental parasitology"
DOI: 10.1016/j.exppara.2018.09.007
Abstract: Hydatigera taeniaeformis (formerly known as Taenia taeniaeformis) is a parasitic tapeworm that has a worldwide distribution. H. taeniaeformis is naturally transmitted between mice and cats and threatens to human health, especially those who are in… read more here.
Keywords: hydatigera taeniaeformis; using deep; sequencing approach; taeniaeformis ... See more keywords
Small RNA and degradome deep sequencing reveals important roles of microRNAs in cotton (Gossypium hirsutum L.) response to root-knot nematode Meloidogyne incognita infection.
Sign Up to like & getrecommendations! Published in 2021 at "Genomics"
DOI: 10.1016/j.ygeno.2021.02.018
Abstract: Investigation of cotton response to nematode infection will allow us to better understand the cotton immune defense mechanism and design a better biotechnological approach for efficiently managing pest nematodes in cotton. In this study, we… read more here.
Keywords: root knot; response; infection; knot nematode ... See more keywords
Deep sequencing and high-throughput analysis of PIWI-associated small RNAs.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2017.05.020
Abstract: Small RNAs are now known to be major regulatory factors of gene expression. Emerging methods based on deep-sequencing have enabled the analysis of small RNA expression in a high-throughput manner, leading to the identification of… read more here.
Keywords: small rna; analysis; small rnas; deep sequencing ... See more keywords
From current knowledge to best practice: A primer on Viral diagnostics using deep sequencing of virus-derived small interfering RNAs (vsiRNAs) in infected plants.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.10.009
Abstract: Plants have evolved many defense strategies for combating viral infections. One major surveillance strategy adopted by them is manipulating viral sequences to generate distinct small RNA products via Dicer-like enzymes (DCL), and thereby restricting virus… read more here.
Keywords: virus derived; using deep; derived small; small interfering ... See more keywords