Development of a general defined medium for Pichia pastoris
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26440
Abstract: Pichia pastoris is widely used as a host for recombinant protein production. More than 500 proteins have been expressed in the organism at a variety of cultivation scales, from small shake flasks to large bioreactors.… read more here.
Keywords: medium; defined medium; pichia pastoris; development ... See more keywords
A comparison of human mesenchymal stem cell osteogenesis in poly(ethylene glycol) hydrogels as a function of MMP‐sensitive crosslinker and crosslink density in chemically defined medium
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26957
Abstract: This study investigated osteogenesis of human mesenchymal stem cells encapsulated in matrix‐metalloproteinase (MMP)‐sensitive poly(ethylene glycol) (PEG) hydrogels in chemically defined medium (10 ng/ml bone morphogenic factor‐2). Thiol‐norbornene photoclick hydrogels were formed with CRGDS and crosslinkers of… read more here.
Keywords: defined medium; mmp sensitive; osteogenesis; cell ... See more keywords
Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral Vectors in the Chemically Defined Medium.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1084-8_7
Abstract: Much investigation is needed to understand the underlying molecular mechanisms of iPSC reprogramming and to improve this technology. Lentivirus-mediated iPSC reprogramming remains the most effective method to study human pluripotency reprogramming. iPSC production is more… read more here.
Keywords: using gfp; defined medium; human fibroblasts; lentiviral vectors ... See more keywords