Defined serum-free culture of human infant small intestinal organoids with predetermined doses of Wnt3a and R-spondin1 from surgical specimens
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DOI: 10.1007/s00383-021-04957-4
Abstract: Refinement of organoid technology is important for studying physiology and disease of the intestine. We aimed to optimize defined serum-free conditions for human infant small intestinal (SI) organoid culture with predetermined doses of Wnt3a and… read more here.
Keywords: infant small; defined serum; human infant; doses wnt3a ... See more keywords
Defined, serum/feeder-free conditions for expansion and drug screening of primary B-acute lymphoblastic leukemia
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DOI: 10.18632/oncotarget.22466
Abstract: Functional screening for compounds represents a major hurdle in the development of rational therapeutics for B-acute lymphoblastic leukemia (B-ALL). In addition, using cell lines as valid models for evaluating responses to novel drug therapies raises… read more here.
Keywords: expansion; defined serum; lymphoblastic leukemia; drug ... See more keywords
A Newly Developed Chemically Defined Serum-Free Medium Suitable for Human Primary Keratinocyte Culture and Tissue Engineering Applications
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DOI: 10.3390/ijms24031821
Abstract: In our experience, keratinocytes cultured in feeder-free conditions and in commercially available defined and serum-free media cannot be as efficiently massively expanded as their counterparts grown in conventional bovine serum-containing medium, nor can they properly… read more here.
Keywords: free medium; serum free; medium; defined serum ... See more keywords