Generation of Gastrointestinal Organoids from Human Pluripotent Stem Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6949-4_12
Abstract: Over the past several decades, developmental biologists have discovered fundamental mechanisms by which organs form in developing embryos. With this information it is now possible to generate human "organoids" by the stepwise differentiation of human… read more here.
Keywords: human pluripotent; pluripotent stem; definitive endoderm; stem cells ... See more keywords
Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiation.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9477-9_4
Abstract: We have developed a method to bulk culture definitive endoderm cells generated from human iPSCs which can be stored and differentiated to hepatocytes. Human iPSC-derived definitive endoderm cells were sorted based on the expression of… read more here.
Keywords: endoderm; bulk culture; definitive endoderm; derived definitive ... See more keywords
Species-specific rewiring of definitive endoderm developmental gene activation via endogenous retroviruses through TET1-mediated demethylation.
Sign Up to like & getrecommendations! Published in 2022 at "Cell reports"
DOI: 10.2139/ssrn.4099069
Abstract: Transposable elements (TEs) are the major sources of lineage-specific genomic innovation and comprise nearly half of the human genome, but most of their functions remain unclear. Here, we identify that a series of endogenous retroviruses… read more here.
Keywords: definitive endoderm; demethylation; tet1 mediated; specific rewiring ... See more keywords