Simultaneous N-Deglycosylation and Digestion of Complex Samples on S-Traps Enables Efficient Glycosite Hypothesis Generation
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DOI: 10.1021/acsomega.2c08071
Abstract: N-linked glycosylation is an important post-translational modification that is difficult to identify and quantify in traditional bottom-up proteomics experiments. Enzymatic deglycosylation of proteins by peptide:N-glycosidase F (PNGase F) prior to digestion and subsequent mass spectrometry… read more here.
Keywords: deglycosylation digestion; sample preparation; digestion complex; digestion ... See more keywords
Deglycosylation of the Isoflavone C-Glucoside Puerarin by a Combination of Two Recombinant Bacterial Enzymes and 3-Oxo-Glucose
Sign Up to like & getrecommendations! Published in 2020 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.00607-20
Abstract: One important role of the gut microbiota is to metabolize dietary nutrients and supplements such as flavonoid glycosides. Ingested glycosides are metabolized by intestinal bacteria to more-absorbable aglycones and further degradation products that show beneficial… read more here.
Keywords: glucoside; deglycosylation; puerarin; study ... See more keywords
The Clinical Significance of Deglycosylated PD-L1 Level Detection Using 28-8 Monoclonal Antibody in Lung Adenocarcinoma
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DOI: 10.2147/ijgm.s381530
Abstract: Purpose The aim of this study was to explore the clinical significance of deglycosylated PD-L1 level and its correlation with EGFR and ALK mutation in lung adenocarcinoma. Materials and Methods We estimated the intensity of… read more here.
Keywords: lung adenocarcinoma; lung; detection; significance ... See more keywords
TiO2 Simultaneous Enrichment, On-Line Deglycosylation, and Sequential Analysis of Glyco- and Phosphopeptides
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DOI: 10.3389/fchem.2021.703176
Abstract: Reversible protein glycosylation and phosphorylation tightly modulate important cellular processes and are closely involved in pathological processes in a crosstalk dependent manner. Because of their significance and low abundances of glyco- and phosphopeptides, several strategies… read more here.
Keywords: simultaneous enrichment; line deglycosylation; glyco phosphopeptides; deglycosylation ... See more keywords
Liver Involvement in Congenital Disorders of Glycosylation and Deglycosylation
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DOI: 10.3389/fped.2021.696918
Abstract: Background: Congenital disorders of glycosylation (CDG) and NGLY1-CDDG (NGLY1-congenital disorder of deglycosylation) usually represent multisystem (especially neurovisceral) diseases with liver involvement reported in some of them. The aim of the study was to characterize the… read more here.
Keywords: disorders glycosylation; deglycosylation; congenital disorders; liver involvement ... See more keywords
Mechanism-Based Pharmacokinetic Model for the Deglycosylation Kinetics of 20(S)-Ginsenosides Rh2
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DOI: 10.3389/fphar.2022.804377
Abstract: Aim: The 20(S)-ginsenoside Rh2 (Rh2) is being developed as a new antitumor drug. However, to date, little is known about the kinetics of its deglycosylation metabolite (protopanoxadiol) (PPD) following Rh2 administration. The aim of this… read more here.
Keywords: mechanism based; administration; rh2; ppd ... See more keywords