Extraction of Highly Degraded DNA from Ancient Bones and Teeth.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9176-1_4
Abstract: We provide a DNA extraction protocol optimized for the recovery of highly fragmented molecules preserved within bones and teeth. In this method, the hard tissue matrix is degraded using an EDTA/Proteinase K lysis buffer, and… read more here.
Keywords: bones teeth; degraded dna; extraction highly; dna ... See more keywords
Testing a series of modifications on genomic library preparation methods for ancient or degraded DNA.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114193
Abstract: Technological advancements have revolutionized ancient and degraded DNA analysis, moving the field to the Next Generation Sequencing era. One of the advancements, the ancient DNA-oriented high-throughput library preparation methods, enabled the sequencing of more endogenous… read more here.
Keywords: ancient degraded; degraded dna; preparation methods; library preparation ... See more keywords
Dealing with low amounts of degraded DNA: Evaluation of SNP typing of challenging forensic samples by using massive parallel sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Forensic Science International: Genetics Supplement Series"
DOI: 10.1016/j.fsigss.2019.09.032
Abstract: Abstract A set of eighty-two forensic samples with different levels of degradation, as well five in vitro damaged samples were analyzed by the Precision ID Identity Panel. PCR amplifications were performed with scalar amount of… read more here.
Keywords: degraded dna; low amounts; dealing low; forensic samples ... See more keywords
High‐throughput microsatellite genotyping in ecology: improved accuracy, efficiency, standardization and success with low‐quantity and degraded DNA
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DOI: 10.1111/1755-0998.12594
Abstract: Microsatellite markers have played a major role in ecological, evolutionary and conservation research during the past 20 years. However, technical constrains related to the use of capillary electrophoresis and a recent technological revolution that has… read more here.
Keywords: degraded dna; success; microsatellite genotyping; genotyping ecology ... See more keywords
Single-tube library preparation for degraded DNA
Sign Up to like & getrecommendations! Published in 2017 at "Methods in Ecology and Evolution"
DOI: 10.1111/2041-210x.12871
Abstract: In recent years, massive parallel sequencing has revolutionised the study of degraded DNA, thus enabling the field of ancient DNA to evolve into that of paleogenomics. Despite these advances, the recovery and sequencing of degraded… read more here.
Keywords: library preparation; single tube; degraded dna; dna ... See more keywords