Proteasome-mediated protein degradation is enhanced by fusion ubiquitin with unstructured degron.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.05.088
Abstract: Methods to induce proteasomal degradation of unwanted proteins are valuable in biomedical studies and thus receive increasing attention. For efficient degradation, the proteasome requires both a ubiquitin tag, which delivers substrates to the proteasome, and… read more here.
Keywords: degradation; proteasome mediated; degron; ubiquitin ... See more keywords
Structural insights into SMCR8 C-degron recognition by FEM1B.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.04.046
Abstract: C-degrons play critical roles in targeting the receptor proteins of Cullin-RING E3 ligase complexes to initiate protein degradation. FEM1 proteins, including FEM1A, FEM1B, and FEM1C, act as the receptors to specifically recognize Arg/C-degrons to enable… read more here.
Keywords: insights smcr8; degron; smcr8 degron; structural insights ... See more keywords
Recognition of the Diglycine C-End Degron by CRL2KLHDC2 Ubiquitin Ligase.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2018.10.021
Abstract: Aberrant proteins can be deleterious to cells and are cleared by the ubiquitin-proteasome system. A group of C-end degrons that are recognized by specific cullin-RING ubiquitin E3 ligases (CRLs) has recently been identified in some… read more here.
Keywords: diglycine end; degron; end; end degron ... See more keywords
N-Degron Pathways in Plastids.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in plant science"
DOI: 10.1016/j.tplants.2019.06.013
Abstract: Protein amino (N) termini are major determinants of protein stability in the cytosol of eukaryotes and prokaryotes, conceptualized in the N-end rule pathway, lately referred to as N-degron pathways. Here we argue for the existence… read more here.
Keywords: degron; degron pathways; pathways plastids;
Repurposing the Damage Repair Protein Methyl Guanine Methyl Transferase as a Ligand Inducible Fusion Degron.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00771
Abstract: We successfully repurpose the DNA repair protein methylguanine methyltransferase (MGMT) as an inducible degron for protein fusions. MGMT is a suicide protein that removes alkyl groups from the O6 position of guanine (O6G) and is… read more here.
Keywords: fusion; degron; repurposing damage; guanine ... See more keywords
Aptamer and N-Degron Ensemble (AptaGron) as a Target Protein Degradation Strategy
Sign Up to like & getrecommendations! Published in 2024 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.4c00536
Abstract: Target protein degradation (TPD) is a promising strategy for catalytic downregulation of target proteins through various cellular proteolytic pathways. Despite numerous reports on novel TPD mechanisms, the discovery of target-specific ligands remains a major challenge.… read more here.
Keywords: aptagron; protein degradation; target protein; target ... See more keywords
Orthogonal IMiD-Degron Pairs Induce Selective Protein Degradation in Cells
Sign Up to like & getrecommendations! Published in 2025 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.5c00751
Abstract: Immunomodulatory imide drugs (IMiDs), including thalidomide, lenalidomide, and pomalidomide, can be used to induce degradation of a protein of interest that is fused to a short degron motif, which often comprises a zinc finger (ZF).… read more here.
Keywords: induce degradation; degradation; orthogonal imid; protein ... See more keywords
Degron-Controlled Protein Degradation in Escherichia coli: New Approaches and Parameters.
Sign Up to like & getrecommendations! Published in 2024 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.3c00768
Abstract: Protein degron tags have proven to be uniquely useful for the characterization of gene function. Degrons can mediate quick depletion, usually within minutes, of a protein of interest, allowing researchers to characterize cellular responses to… read more here.
Keywords: escherichia coli; system; degradation; protein degradation ... See more keywords
Mechanism of Ψ-Pro/C-degron recognition by the CRL2FEM1B ubiquitin ligase
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-47890-5
Abstract: The E3 ligase-degron interaction determines the specificity of the ubiquitin‒proteasome system. We recently discovered that FEM1B, a substrate receptor of Cullin 2-RING ligase (CRL2), recognizes C-degrons containing a C-terminal proline. By solving several cryo-EM structures… read more here.
Keywords: ligase; terminal proline; recognition; crl2fem1b ... See more keywords
Molecular insights into degron recognition by CRL5ASB7 ubiquitin ligase
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-50556-x
Abstract: The ankyrin (ANK) SOCS box (ASB) family, encompassing ASB1–18, is the largest group of substrate receptors of cullin 5 Ring E3 ubiquitin ligase. Nonetheless, the mechanism of substrate recognition by ASB family proteins has remained… read more here.
Keywords: recognition; molecular insights; ubiquitin ligase; insights degron ... See more keywords
Molecular basis for ubiquitin ligase CRL2FEM1C-mediated recognition of C-degron
Sign Up to like & getrecommendations! Published in 2021 at "Nature Chemical Biology"
DOI: 10.1038/s41589-020-00703-4
Abstract: Proteome integrity depends on the ubiquitin–proteasome system to degrade unwanted or abnormal proteins. In addition to the N-degrons, C-terminal residues of proteins can also serve as degradation signals (C-degrons) that are recognized by specific cullin-RING… read more here.
Keywords: arg degron; terminal arginine; degron; molecular basis ... See more keywords