Cloning, expression, and biochemical characterization of a novel NADP+-dependent 7α-hydroxysteroid dehydrogenase from Clostridium difficile and its application for the oxidation of bile acids.
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DOI: 10.1016/j.enzmictec.2016.12.006
Abstract: A gene encoding a novel 7α-specific NADP+-dependent hydroxysteroid dehydrogenase from Clostridium difficile was cloned and heterologously expressed in Escherichia coli. The enzyme was purified using an N-terminal hexa-his-tag and biochemically characterized. The optimum temperature is… read more here.
Keywords: clostridium difficile; nadp dependent; clostridium; dehydrogenase clostridium ... See more keywords