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Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1597-3_18
Abstract: Loss-of-function experiments are essential for the functional investigation of cis-regulatory elements (CREs), such as transcriptional enhancers. This can be achieved with CRISPR-Cas9 using pairs of single guide RNAs (sgRNAs) to target the flanking regions of…
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Keywords:
crispr cas9;
deletion regulatory;
one crispr;
regulatory elements ... See more keywords