Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7546-4_18
Abstract: We present an efficient and organized method of lithium acetate and polyethylene glycol-based transformation of plasmid DNA into the commercially available collection of Schizosaccharomyces pombe with single-gene deletions. We also describe how to prepare a… read more here.
Keywords: schizosaccharomyces pombe; transformation; deletion; deletion strains ... See more keywords
Facilitating the enzymatic conversion of lysineto cadaverine in engineered Escherichia coli with metabolic regulation by genes deletion
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2020.107514
Abstract: Abstract Cadaverine, also known as 1,5-diaminopentane, is a biogenic amine particularly used for the synthesis of nylon. Cadaverine can be efficiently synthesized through decarboxylation of lysine in microorganisms. However, the respective metabolic pathways in Escherichia… read more here.
Keywords: escherichia coli; enzymatic conversion; deletion; cadaverine ... See more keywords
Profiling Yeast Deletion Strains Using Sample Multiplexing and Network-Based Analyses.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00137
Abstract: The yeast, Saccharomyces cerevisiae, is a widely used model system for investigating conserved biological functions and pathways. Advancements in sample multiplexing have facilitated the study of the yeast proteome, yet many yeast proteins remain uncharacterized… read more here.
Keywords: sample multiplexing; network based; deletion; deletion strains ... See more keywords
Identification of Key Genes during Ca2+-Induced Genetic Transformation in Escherichia coli by Combining Multi-Omics and Gene Knockout Techniques
Sign Up to like & getrecommendations! Published in 2022 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.00587-22
Abstract: Using transcriptome and proteome techniques and association analysis, we identified several key genes involved in the formation of Ca2+-mediated E. coli DH5α competent cells. We used Red homologous recombination technology to construct three single-gene deletion… read more here.
Keywords: key genes; deletion strains; gene; transformation ... See more keywords
Synthetic Genetic Interactions Reveal a Dense and Cryptic Regulatory Network of Small Noncoding RNAs in Escherichia coli
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DOI: 10.1128/mbio.01225-22
Abstract: sRNAs have long been purported to be a critical mechanism by which bacteria respond to stress; however, uncovering growth phenotypes for sRNA deletion strains in E. coli and related bacteria has proven particularly challenging. In… read more here.
Keywords: growth; deletion; deletion strains; srna deletion ... See more keywords
A Triplex PCR Method for Distinguishing the Wild-Type African Swine Fever Virus From the Deletion Strains by Detecting the Gene Insertion
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Veterinary Science"
DOI: 10.3389/fvets.2022.921907
Abstract: To date, there is no effective vaccine or antiviral therapy available to prevent or treat African swine fever virus (ASFV) infections. ASFV gene deletion strains have been proposed as promising anti-ASFV vaccine candidates. In recent… read more here.
Keywords: deletion strains; pcr; gene; virus ... See more keywords