Harnessing nanoparticles for the efficient delivery of the CRISPR/Cas9 system
Sign Up to like & getrecommendations! Published in 2020 at "Nano Today"
DOI: 10.1016/j.nantod.2020.100895
Abstract: Abstract Exploiting bacterial DNA-acting enzymes and expanding their repertoire for genome engineering have been major technological and conceptual advances in molecular biology over the last decade. The CRISPR-Cas9 system offers many attractive superiorities, such as… read more here.
Keywords: crispr cas9; delivery crispr; cas9; cas9 system ... See more keywords
Efficient Intracellular Delivery of CRISPR-Cas Ribonucleoproteins through Receptor Mediated Endocytosis.
Sign Up to like & getrecommendations! Published in 2019 at "ACS chemical biology"
DOI: 10.1021/acschembio.9b00116
Abstract: We recently reported a new delivery system harnessing surface receptors for targeted uptake of CRISPR-Cas9 ribonucleoprotein into mammalian cells (Rouet et al., JACS 2018). For this purpose, Cas9 protein was labeled with the small molecule… read more here.
Keywords: delivery crispr; delivery; efficient intracellular; intracellular delivery ... See more keywords
P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00465
Abstract: The discovery of clustered, regularly interspaced, short palindromic repeats (CRISPR) and the Cas9 RNA-guided nuclease provides unprecedented opportunities to selectively kill specific populations or species of bacteria. However, the use of CRISPR-Cas9 to clear bacterial… read more here.
Keywords: crispr cas9; shigella flexneri; delivery crispr; delivery ... See more keywords
CRISPR/Cas9 gene editing of brain cancer stem cells using lipid-based nano-delivery
Sign Up to like & getrecommendations! Published in 2019 at "Neuro-Oncology"
DOI: 10.1093/neuonc/noz167.029
Abstract: Despite advances in cancer therapy glioblastoma (GBM) remains one of the deadliest brain tumours. Effective therapy is restricted by the presence of multiple resistance mechanisms. Physical barriers such as the blood-brain barrier limit the brain… read more here.
Keywords: crispr cas9; delivery crispr; brain; cas9 ... See more keywords