An Efficient Bead‐captured Denaturation Method for Preparing Long Single‐stranded DNA
Sign Up to like & getrecommendations! Published in 2017 at "Journal of The Chinese Chemical Society"
DOI: 10.1002/jccs.201700142
Abstract: Previous methods to prepare single-stranded DNA (ssDNA) substrates are limited to short DNA lengths and inefficient. We have developed an efficient and rapid method to prepare long ssDNA substrates (up to 4000 nt) based on the… read more here.
Keywords: single stranded; dna; ssdna; bead captured ... See more keywords
HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2016.10.010
Abstract: Glucosinolates are plant secondary metabolites abundant in Brassica vegetables that are substrates for the enzyme myrosinase, a thioglucoside hydrolase. Enzyme-mediated hydrolysis of glucosinolates forms several organic products, including isothiocyanates (ITCs) that have been explored for… read more here.
Keywords: denaturation; multiple reaction; non natural; hplc based ... See more keywords
FTIR study of synthesised ovalbumin nanoparticles.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2021.114456
Abstract: Ovalbumin particles are reduced to nano size using heat treatment techniques. Their structural patterns in their native state and in their pH denatured state were attempted. Denaturation is also a part of conformation and hence… read more here.
Keywords: study synthesised; synthesised ovalbumin; ovalbumin nanoparticles; ftir study ... See more keywords
Molecular assessment of collagen denaturation in decellularized tissues using a collagen hybridizing peptide
Sign Up to like & getrecommendations! Published in 2017 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2017.01.079
Abstract: Decellularized extracellular matrix (ECM) derived from tissues and organs are emerging as important scaffold materials for regenerative medicine. Many believe that preservation of the native ECM structure during decellularization is highly desirable. However, because effective… read more here.
Keywords: collagen; collagen hybridizing; denaturation; decellularized tissues ... See more keywords
Filtration of protein-based solutions with ceramic ultrafiltration membrane. Study of selectivity, adsorption, and protein denaturation
Sign Up to like & getrecommendations! Published in 2019 at "Comptes Rendus Chimie"
DOI: 10.1016/j.crci.2018.09.011
Abstract: Abstract Proteins are widely used in food or pharmaceutical industry and are often pumped in complex flow systems. Their biological activity is intrinsically related to their conformation in solution. In this work, we studied the… read more here.
Keywords: denaturation; membrane; ultrafiltration membrane; ceramic ultrafiltration ... See more keywords
Studying the denaturation of bovine serum albumin by a novel approach of difference-UV analysis.
Sign Up to like & getrecommendations! Published in 2017 at "Food chemistry"
DOI: 10.1016/j.foodchem.2016.07.133
Abstract: A novel approach in the analysis of difference-UV spectrophotometric data for determining the heat denaturation degree of bovine serum albumin (BSA) was assessed. Five different parameters of difference-UV spectra were obtained by subtracting spectra of… read more here.
Keywords: difference; heat denaturation; novel approach; analysis ... See more keywords
Gastrophysics: Statistical thermodynamics of biomolecular denaturation and gelation from the Kirkwood-Buff theory towards the understanding of tofu
Sign Up to like & getrecommendations! Published in 2017 at "Food Hydrocolloids"
DOI: 10.1016/j.foodhyd.2016.07.022
Abstract: Sugars, alcohols, or salts, when added to food, affects the heat denaturation of proteins and the sol-gel transition of macromolecules. Such an effect of cosolvents has long been known and exploited; yet understanding how they… read more here.
Keywords: kirkwood buff; theory; thermodynamics; buff theory ... See more keywords
Shear-induced structural changes and denaturation of bovine immunoglobulin G and serum albumin at different temperatures
Sign Up to like & getrecommendations! Published in 2022 at "Food Hydrocolloids"
DOI: 10.1016/j.foodhyd.2021.107283
Abstract: Abstract Shearing can modify native properties of proteins alone or in combination with heating. Effects of shearing on secondary structure, extent of denaturation and interactions of immunoglobulin G (IgG) and bovine serum albumin (BSA) were… read more here.
Keywords: bsa; bovine; igg bsa; denaturation ... See more keywords
Estimation of thermodynamic stability of human carbonic anhydrase IX from urea-induced denaturation and MD simulation studies.
Sign Up to like & getrecommendations! Published in 2017 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2017.07.010
Abstract: Carbonic anhydrase IX (CAIX) is a transmembrane glycoprotein, overexpressed in cancer cells under hypoxia condition. In cancerous cells, CAIX plays an important role to combat the deleterious effects of a high rate of glycolytic metabolism.… read more here.
Keywords: carbonic anhydrase; induced denaturation; denaturation; urea induced ... See more keywords
Glutaraldehyde crosslinked and alkaline denaturation induced self association of haemoglobin to design nanocarriers for In vitro release of insulin in simulated gastrointestinal fluids (SGFs)
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Drug Delivery Science and Technology"
DOI: 10.1016/j.jddst.2017.12.004
Abstract: Abstract Adopting a novel synthetic route the haemoglobin nanoparticles were prepared by its simultaneous microemulsion mediated crosslinking with glutaraldehyde and alkaline induced denaturation. The as prepared nanoparticles were characterized by techniques like Fourier transform infrared… read more here.
Keywords: insulin; denaturation; insulin simulated; haemoglobin ... See more keywords
Analysis of color changes in chicken egg yolks and whites based on degree of thermal protein denaturation during ohmic heating and water bath treatment
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Food Engineering"
DOI: 10.1016/j.jfoodeng.2017.11.024
Abstract: Abstract Raw eggs were separated into yolks and whites before thermal treatment. An ohmic heating process at 20 kHz and 30 V was used for egg-yolk samples, and color changes were monitored with a computer vision system.… read more here.
Keywords: color; yolks whites; color changes; egg ... See more keywords