Terminal deoxynucleotidyl transferase and CD84 identify human multi-potent lymphoid progenitors
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-49883-w
Abstract: Lymphoid specification in human hematopoietic progenitors is not fully understood. To better associate lymphoid identity with protein-level cell features, we conduct a highly multiplexed single-cell proteomic screen on human bone marrow progenitors. This screen identifies… read more here.
Keywords: terminal deoxynucleotidyl; deoxynucleotidyl transferase; multi; cd84 ... See more keywords
Terminal deoxynucleotidyl transferase-mediated formation of protein binding polynucleotides
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa1263
Abstract: Abstract Terminal deoxynucleotidyl transferase (TdT) enzyme plays an integral part in the V(D)J recombination, allowing for the huge diversity in expression of immunoglobulins and T-cell receptors within lymphocytes, through their unique ability to incorporate single… read more here.
Keywords: protein; transferase mediated; deoxynucleotidyl transferase; mediated formation ... See more keywords
Evolving a terminal deoxynucleotidyl transferase for commercial enzymatic DNA synthesis
Sign Up to like & getrecommendations! Published in 2025 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaf115
Abstract: Abstract Enzymatic DNA synthesis, using stepwise nucleotide addition catalyzed by template-independent polymerases, promises higher efficiency, quality, and sustainability than today’s industry-standard phosphoramidite-based processes. We report the directed evolution of a terminal deoxynucleotidyl transferase that uses… read more here.
Keywords: terminal deoxynucleotidyl; deoxynucleotidyl transferase; dna synthesis; enzymatic dna ... See more keywords