A Conserved Histidine Residue Drives Extein Dependence in an Enhanced Atypically Split Intein.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c08985
Abstract: Split intein-mediated protein trans-splicing (PTS) is widely applied in chemical biology and biotechnology to carry out traceless and specific protein ligation. However, the external residues immediately flanking the intein (exteins) can reduce the splicing rate,… read more here.
Keywords: dependence; dependence enhanced; atypically split; extein dependence ... See more keywords