Getting to know the neighborhood: using proximity-dependent biotinylation to characterize protein complexes and map organelles.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2018.10.017
Abstract: The use of proximity-dependent biotinylation approaches combined with mass spectrometry (e.g. BioID and APEX) has revolutionized the study of protein-protein interactions and organellar proteomics. These powerful techniques are based on the fusion of an enzyme… read more here.
Keywords: protein; dependent biotinylation; neighborhood; proximity dependent ... See more keywords
Proximity-Dependent Biotinylation Approaches to Explore the Dynamic Compartmentalized Proteome
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DOI: 10.3389/fmolb.2022.852911
Abstract: In recent years, proximity-dependent biotinylation approaches, including BioID, APEX, and their derivatives, have been widely used to define the compositions of organelles and other structures in cultured cells and model organisms. The associations between specific… read more here.
Keywords: biotinylation approaches; proximity dependent; proximity; approaches explore ... See more keywords
Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cellular Neuroscience"
DOI: 10.3389/fncel.2022.801644
Abstract: Proximity-dependent biotinylation techniques have been gaining wide applications in the systematic analysis of protein-protein interactions (PPIs) on a proteome-wide scale in living cells. The engineered biotin ligase TurboID is among the most widely adopted given… read more here.
Keywords: biotinylation; proximity dependent; dependent biotinylation; biotinylation living ... See more keywords