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   Articles with "dependent biotinylation" as a keyword



Getting to know the neighborhood: using proximity-dependent biotinylation to characterize protein complexes and map organelles.

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recommendations! Published in 2019 at "Current opinion in chemical biology"

DOI: 10.1016/j.cbpa.2018.10.017

Abstract: The use of proximity-dependent biotinylation approaches combined with mass spectrometry (e.g. BioID and APEX) has revolutionized the study of protein-protein interactions and organellar proteomics. These powerful techniques are based on the fusion of an enzyme… read more here.

Keywords: protein; dependent biotinylation; neighborhood; proximity dependent ... See more keywords

Exploring Options for Proximity-Dependent Biotinylation Experiments: Comparative Analysis of Labeling Enzymes and Affinity Purification Resins

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recommendations! Published in 2024 at "Journal of Proteome Research"

DOI: 10.1021/acs.jproteome.3c00908

Abstract: Proximity-dependent biotinylation (PDB) techniques provide information about the molecular neighborhood of a protein of interest, yielding insights into its function and localization. Here, we assessed how different labeling enzymes and streptavidin resins influence PDB results.… read more here.

Keywords: dependent biotinylation; proximity dependent; resin; labeling enzymes ... See more keywords

An APEX2-based proximity-dependent biotinylation assay with temporal specificity to study protein interactions during autophagy in the yeast Saccharomyces cerevisiae

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recommendations! Published in 2024 at "Autophagy"

DOI: 10.1080/15548627.2024.2366749

Abstract: ABSTRACT Autophagosome biogenesis is a complex process orchestrated by dynamic interactions between Atg (autophagy-related) proteins and characterized by the turnover of specific cargoes, which can differ over time and depending on how autophagy is stimulated.… read more here.

Keywords: protein; temporal specificity; dependent biotinylation; proximity ... See more keywords

Proximity-Dependent Biotinylation Approaches to Explore the Dynamic Compartmentalized Proteome

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recommendations! Published in 2022 at "Frontiers in Molecular Biosciences"

DOI: 10.3389/fmolb.2022.852911

Abstract: In recent years, proximity-dependent biotinylation approaches, including BioID, APEX, and their derivatives, have been widely used to define the compositions of organelles and other structures in cultured cells and model organisms. The associations between specific… read more here.

Keywords: biotinylation approaches; proximity dependent; proximity; approaches explore ... See more keywords

Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells

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recommendations! Published in 2022 at "Frontiers in Cellular Neuroscience"

DOI: 10.3389/fncel.2022.801644

Abstract: Proximity-dependent biotinylation techniques have been gaining wide applications in the systematic analysis of protein-protein interactions (PPIs) on a proteome-wide scale in living cells. The engineered biotin ligase TurboID is among the most widely adopted given… read more here.

Keywords: biotinylation; proximity dependent; dependent biotinylation; biotinylation living ... See more keywords