Producing defucosylated antibodies with enhanced in vitro antibody‐dependent cellular cytotoxicity via FUT8 knockout CHO‐S cells
Sign Up to like & getrecommendations! Published in 2017 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201600255
Abstract: To engineer a host cell line that produces defucosylated mAbs with superior antibody‐dependent cellular cytotoxicity, we disrupted α‐1, 6 fucosyltransferase (FUT8) gene in CHO‐S (CHO is Chinese hamster ovary) cells by clustered regularly interspaced short… read more here.
Keywords: dependent cellular; knockout; antibody dependent; cellular cytotoxicity ... See more keywords
Development of a novel reporter gene assay to evaluate antibody-dependent cellular phagocytosis for anti-CD20 therapeutic antibodies.
Sign Up to like & getrecommendations! Published in 2021 at "International immunopharmacology"
DOI: 10.1016/j.intimp.2021.108112
Abstract: More than 100 monoclonal antibodies (mAbs) have been approved by FDA. The mechanism of action (MoA) involves in neutralization of a specific target via the Fab region and Fc effector functions through Fc region, while… read more here.
Keywords: antibody; adcp; cellular phagocytosis; antibody dependent ... See more keywords
Targeting the Late Stage of HIV-1 Entry for Antibody-Dependent Cellular Cytotoxicity: Structural Basis for Env Epitopes in the C11 Region.
Sign Up to like & getrecommendations! Published in 2017 at "Structure"
DOI: 10.1016/j.str.2017.09.009
Abstract: Antibodies can have an impact on HIV-1 infection in multiple ways, including antibody-dependent cellular cytotoxicity (ADCC), a correlate of protection observed in the RV144 vaccine trial. One of the most potent ADCC-inducing epitopes on HIV-1… read more here.
Keywords: antibody; antibody dependent; entry; dependent cellular ... See more keywords
β-Pix-dependent cellular protrusions propel collective mesoderm migration in the mouse embryo
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-19889-1
Abstract: Coordinated directional migration of cells in the mesoderm layer of the early embryo is essential for organization of the body plan. Here we show that mesoderm organization in mouse embryos depends on β-Pix (Arhgef7), a… read more here.
Keywords: cellular protrusions; pix dependent; migration; dependent cellular ... See more keywords
Small CD4 mimetics sensitize HIV-1-infected macrophages to antibody-dependent cellular cytotoxicity
Sign Up to like & getrecommendations! Published in 2022 at "Cell reports"
DOI: 10.1101/2022.06.30.498265
Abstract: HIV-1 Envelope (Env) conformation determines the susceptibility of infected CD4+ T cells to Antibody Dependent Cellular Cytotoxicity (ADCC). Upon interaction with CD4, Env adopts more “open” conformations, exposing ADCC epitopes. HIV-1 limits Env-CD4 interaction and… read more here.
Keywords: antibody dependent; cd4; infected macrophages; cellular cytotoxicity ... See more keywords
Development of a reporter gene assay for antibody dependent cellular cytotoxicity activity determination of anti‐rabies virus glycoprotein antibodies
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology and Immunology"
DOI: 10.1111/1348-0421.13036
Abstract: Rabies is a viral disease that is nearly 100% fatal once clinical signs and symptoms develop. Post‐exposure prophylaxis can efficiently prevent rabies, and antibody (Ab) induction by vaccination or passive immunization of human rabies immunoglobulin… read more here.
Keywords: antibody dependent; reporter gene; activity; dependent cellular ... See more keywords
Modulating antibody‐dependent cellular cytotoxicity of epidermal growth factor receptor‐specific heavy‐chain antibodies through hinge engineering
Sign Up to like & getrecommendations! Published in 2019 at "Immunology and Cell Biology"
DOI: 10.1111/imcb.12238
Abstract: Human IgG1 and IgG3 antibodies (Abs) can mediate Ab‐dependent cellular cytotoxicity (ADCC), and engineering of the Ab Fc (point mutation; defucosylation) has been shown to affect ADCC by modulating affinity for FcRγIIIa. In the absence… read more here.
Keywords: adcc; heavy chain; hinge; dependent cellular ... See more keywords
Uninfected Bystander Cells Impact the Measurement of HIV-Specific Antibody-Dependent Cellular Cytotoxicity Responses
Sign Up to like & getrecommendations! Published in 2018 at "mBio"
DOI: 10.1128/mbio.00358-18
Abstract: ABSTRACT The conformation of the HIV-1 envelope glycoprotein (Env) substantially impacts antibody recognition and antibody-dependent cellular cytotoxicity (ADCC) responses. In the absence of the CD4 receptor at the cell surface, primary Envs sample a “closed”… read more here.
Keywords: hiv; dependent cellular; antibody dependent; bystander cells ... See more keywords
Antibody-dependent cellular cytotoxicity, infected cell binding and neutralization by antibodies to the SIV envelope glycoprotein.
Sign Up to like & getrecommendations! Published in 2023 at "PLoS pathogens"
DOI: 10.1371/journal.ppat.1011407
Abstract: Antibodies specific for diverse epitopes of the simian immunodeficiency virus envelope glycoprotein (SIV Env) have been isolated from rhesus macaques to provide physiologically relevant reagents for investigating antibody-mediated protection in this species as a nonhuman… read more here.
Keywords: antibody dependent; adcc; envelope glycoprotein; neutralization ... See more keywords
An Engineered Human Fc variant With Exquisite Selectivity for FcγRIIIaV158 Reveals That Ligation of FcγRIIIa Mediates Potent Antibody Dependent Cellular Phagocytosis With GM-CSF-Differentiated Macrophages
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2019.00562
Abstract: IgG antibodies mediate the clearance of target cells via the engagement of Fc gamma receptors (FcγRs) on effector cells by eliciting antibody-dependent cellular cytotoxicity and phagocytosis (ADCC and ADCP, respectively). Because (i) the IgG Fc… read more here.
Keywords: riiiav158; hfc; antibody dependent; dependent cellular ... See more keywords
Influenza and Antibody-Dependent Cellular Cytotoxicity
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2019.01457
Abstract: Despite the availability of yearly vaccinations, influenza continues to cause seasonal, and pandemic rises in illness and death. An error prone replication mechanism results in antigenic drift and viral escape from immune pressure, and recombination… read more here.
Keywords: antibody; cellular cytotoxicity; antibody dependent; dependent cellular ... See more keywords