A Nucleophilic Activity‐Based Probe Enables Profiling of PLP‐Dependent Enzymes
Sign Up to like & getrecommendations! Published in 2023 at "ChemBioChem"
DOI: 10.1002/cbic.202200669
Abstract: PLP‐dependent enzymes represent an important class of highly “druggable” enzymes that perform a wide array of critical reactions to support all organisms. Inhibition of individual members of this family of enzymes has been validated as… read more here.
Keywords: dependent enzymes; plp dependent; nucleophilic activity; activity based ... See more keywords
Discovering radical-dependent enzymes in the human gut microbiota.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2018.09.011
Abstract: Human gut microbes have a tremendous impact on human health, in part due to their unique chemical capabilities. In the anoxic environment of the healthy human gut, many important microbial metabolic transformations are performed by… read more here.
Keywords: radical dependent; biology; dependent enzymes; human gut ... See more keywords
Direct Detection of Products from S-Adenosylmethionine-Dependent Enzymes Using a Competitive Fluorescence Polarization Assay.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b03556
Abstract: S-Adenosylmethionine (AdoMet)-dependent methyltransferases (MTases) are an essential superfamily of enzymes that catalyze the transfer of a methyl group to several biomolecules. Alterations in the methylation of cellular components crucially impact vital biological processes, making MTases… read more here.
Keywords: dependent enzymes; competitive fluorescence; fluorescence polarization; polarization assay ... See more keywords
Catalytic Roles of Coenzyme Pyridoxal-5'-phosphate (PLP) in PLP-dependent Enzymes: Reaction Pathway for Methionine-γ-lyase-catalyzed L-methionine Depletion.
Sign Up to like & getrecommendations! Published in 2020 at "ACS catalysis"
DOI: 10.1021/acscatal.9b03907
Abstract: Pyridoxal-5'-phosphate (PLP), the active form of vitamin B6, is an important and versatile coenzyme involved in a variety of enzymatic reactions, accounting for about 4% of all classified activities. However, the detailed catalytic reaction pathways… read more here.
Keywords: plp; phosphate plp; pyridoxal phosphate; dependent enzymes ... See more keywords
Inhibition of Thiamine Diphosphate-Dependent Enzymes by Triazole-Based Thiamine Analogues
Sign Up to like & getrecommendations! Published in 2023 at "ACS Medicinal Chemistry Letters"
DOI: 10.1021/acsmedchemlett.3c00047
Abstract: Thiamine is metabolized into the coenzyme thiamine diphosphate (ThDP). Interrupting thiamine utilization leads to disease states. Oxythiamine, a thiamine analogue, is metabolized into oxythiamine diphosphate (OxThDP), which inhibits ThDP-dependent enzymes. Oxythiamine has been used to… read more here.
Keywords: thiamine; thiamine diphosphate; dependent enzymes; thiamine utilization ... See more keywords
Complete Biosynthetic Pathway of the Phosphonate Phosphonothrixin: Two Distinct Thiamine Diphosphate-Dependent Enzymes Divide the Work to Form a C-C Bond.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c06546
Abstract: Phosphonates often exhibit biological activities by mimicking the phosphates and carboxylates of biological molecules. The phosphonate phosphonothrixin (PTX), produced by the soil-dwelling bacterium Saccharothrix sp. ST-888, exhibits herbicidal activity. In this study, we propose a… read more here.
Keywords: thiamine diphosphate; dependent enzymes; phosphonate phosphonothrixin; complete biosynthetic ... See more keywords
Pyridoxal 5′-Phosphate-Dependent Enzymes at the Crossroads of Host–Microbe Tryptophan Metabolism
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms21165823
Abstract: The chemical processes taking place in humans intersects the myriad of metabolic pathways occurring in commensal microorganisms that colonize the body to generate a complex biochemical network that regulates multiple aspects of human life. The… read more here.
Keywords: trp metabolism; metabolism; pyridoxal phosphate; host ... See more keywords
Theoretical Studies of Nickel-Dependent Enzymes
Sign Up to like & getrecommendations! Published in 2019 at "Inorganics"
DOI: 10.3390/inorganics7080095
Abstract: The advancements of quantum chemical methods and computer power allow detailed mechanistic investigations of metalloenzymes. In particular, both quantum chemical cluster and combined QM/MM approaches have been used, which have been proven to successfully complement… read more here.
Keywords: studies nickel; theoretical studies; nickel dependent; dependent enzymes ... See more keywords