Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs.
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DOI: 10.1093/nar/gkad323
Abstract: Streptococcus pyogenes Cas9 (SpCas9) nuclease exhibits considerable position-dependent sequence preferences. The reason behind these preferences is not well understood and is difficult to rationalise, since the protein establishes interactions with the target-spacer duplex in a… read more here.
Keywords: spacer motifs; dependent sequence; sequence; position dependent ... See more keywords