Quantitative and Multiplex Detection of Extracellular Vesicle‐Derived MicroRNA via Rolling Circle Amplification within Encoded Hydrogel Microparticles
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202102332
Abstract: Extracellular vesicle‐derived microRNA (EV‐miRNA) represent a promising cancer biomarker for disease diagnosis and monitoring. However, existing techniques to detect EV‐miRNA rely on complex, bias‐prone strategies, and preprocessing steps, making absolute quantification highly challenging. This work… read more here.
Keywords: extracellular vesicle; derived microrna; detection; vesicle derived ... See more keywords
A new CRISPR-derived microRNA sensing mechanism based on Cas12a self-powered and rolling circle transcription (RCT)-unleashed real-time crRNA recruiting.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c00680
Abstract: Current CRISPR-Cas-based nucleic acid sensing methods relying on the pre-assembled Cas-crRNA complexes are gen-erally limited to the detection of protospacer-adjacent motif (PAM)-containing sequences, and nonspecific back-grounds are inevitable. Herein, we propose a new CRISPR-derived microRNA… read more here.
Keywords: crispr; rct; cas12a; crispr derived ... See more keywords