Variable susceptibility of intestinal organoid–derived monolayers to SARS-CoV-2 infection
Sign Up to like & getrecommendations! Published in 2022 at "PLoS Biology"
DOI: 10.1101/2021.07.16.452680
Abstract: Gastrointestinal effects associated with COVID-19 are highly variable for reasons that are not understood. In this study, we used intestinal organoid-derived cultures differentiated from primary human specimens as a model to examine inter-individual variability. Infection… read more here.
Keywords: derived monolayers; intestinal organoid; infection; organoid derived ... See more keywords
Human duodenal organoid‐derived monolayers serve as a suitable barrier model for duodenal tissue
Sign Up to like & getrecommendations! Published in 2022 at "Annals of the New York Academy of Sciences"
DOI: 10.1111/nyas.14804
Abstract: Usually, duodenal barriers are investigated using intestinal cell lines like Caco‐2, which in contrast to native tissue are limited in cell‐type representation. Organoids can consist of all intestinal cell types and are supposed to better… read more here.
Keywords: cell; derived monolayers; barrier; duodenal tissue ... See more keywords
From in vitro development to accessible luminal interface of neonatal bovine-derived intestinal organoids
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DOI: 10.1186/s12917-025-04773-1
Abstract: Intestinal organoids provide physiologically relevant in vitro models that bridge the gap between conventional cell culture and animal studies. Although these systems have been developed for adult cattle, their use in neonatal calves—who are particularly… read more here.
Keywords: organoid derived; derived monolayers; day old; culture ... See more keywords
Establishment of bovine 3D enteroid-derived 2D monolayers
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DOI: 10.1186/s13567-022-01033-0
Abstract: Three-dimensional (3D) intestinal enteroids are powerful in vitro models for studying intestinal biology. However, due to their closed structure direct access to the apical surface is impeded, limiting high-throughput applications of exogenous compounds and pathogens.… read more here.
Keywords: establishment bovine; derived monolayers; cell; enteroid derived ... See more keywords
Validating Enteroid-Derived Monolayers from Murine Gut Organoids for Toxicological Testing of Inorganic Particles: Proof-of-Concept with Food-Grade Titanium Dioxide
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DOI: 10.3390/ijms25052635
Abstract: Human exposure to foodborne inorganic nanoparticles (NPs) is a growing concern. However, identifying potential hazards linked to NP ingestion often requires long-term exposure in animals. Owing these constraints, intestinal organoids are a promising alternative to… read more here.
Keywords: enteroid derived; derived monolayers; food grade; validating enteroid ... See more keywords
Differential Colonization and Mucus Ultrastructure Visualization in Bovine Ileal and Rectal Organoid-Derived Monolayers Exposed to Enterohemorrhagic Escherichia coli
Sign Up to like & getrecommendations! Published in 2024 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms25094914
Abstract: Enterohemorrhagic Escherichia coli (EHEC) is a critical public health concern due to its role in severe gastrointestinal illnesses in humans, including hemorrhagic colitis and the life-threatening hemolytic uremic syndrome. While highly pathogenic to humans, cattle,… read more here.
Keywords: escherichia coli; organoid derived; derived monolayers; mucus ... See more keywords