Articles with "design cloning" as a keyword



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In silico design, cloning, expression and immunologic evaluation of ED fusion protein of NT H. influenzae.

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Published in 2017 at "Microbial pathogenesis"

DOI: 10.1016/j.micpath.2017.11.007

Abstract: Infections due to nontypeable Haemophilus influenzae (NTHi) are important causes of child mortality throughout the world. Given the lack of effective vaccines for these strains and the spread and prevalence of these infections in the… read more here.

Keywords: silico design; fusion protein; design cloning; design ... See more keywords
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A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector

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Published in 2017 at "BMC Biotechnology"

DOI: 10.1186/s12896-017-0341-x

Abstract: BackgroundShort hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing the ability to infect most mammalian cell types… read more here.

Keywords: vector; design; streamlined method; tet plko ... See more keywords